Xeno-Free Hydrogels for 3D Cell Culture

Over 255+ publications and references.

VitroGel® is a biofunctional animal-free hydrogel for 3D cell culture.  Achieve 3D cell culture assays in less than 20 minutes with consistent results.

Xeno-Free Hydrogel

Room Temp Stable

Easy Cell Harvesting

Lot-to-Lot Consistency

Adaptable for
High-Throughput

APPLICATION NOTESRESEARCH HIGHLIGHTS

Evaluating Spheroid Invasion with VitroGel® Hydrogel Matrix in VitroPrime™ Ultra-Low Attachment, U-Bottom, 96-well Plate

Introduction Spheroid invasion assays offer a physiologically relevant three-dimensional (3D) in vitro model for studying cancer cell behavior, surpassing the limitations of traditional two-dimensional (2D) cultures [1]. Spheroids are compact cell aggregates that [...]

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VitroGel® at the Frontlines: Enabling 3D Modeling of Venous Invasion in Pancreatic Cancer

This study leveraged VitroGel®’s hydrogel system to model the tumor microenvironment and uncover the role of TIMP1 in driving venous invasion in pancreatic cancer. Hydrogel: VitroGel® Hydrogel Matrix (VHM01) Pancreatic ductal adenocarcinoma (PDAC) [...]

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Bringing the Brain to the Bench: A Microfluidic BBB Model for Tumor Interaction Studies

VitroGel® IKVAV was a key component that enabled stable vessel formation, proper cellular organization and functional BBB behavior in the 3D microfluidic model. Hydrogel: VitroGel® IKVAV (Cat. No: TWG007) The blood-brain barrier (BBB) [...]

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Advancing Bone Regeneration with Xeno-free VitroGel® System

VitroGel® Hydrogel Matrix enables the controlled delivery of bioactive Qu-SeNPs, accelerating bone defect healing and enhancing therapeutic potential for regenerative medicine. Hydrogel: VitroGel® Hydrogel Matrix (Cat. No: VHM01) The development of biomimetic hydrogels [...]

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VitroGel®-Enhanced 3D Culture Models Reveal the Potential of Basroparib in Overcoming MEK Inhibitor Resistance in KRAS-Mutated Colorectal Cancer

VitroGel® supports 3D spheroid models for evaluating basroparib’s efficacy in overcoming MEK inhibitor resistance in KRAS-mutated colorectal cancer. Hydrogel: VitroGel® RGD (Cat. No: TWG003) The study on basroparib’s role in overcoming MEK inhibitor [...]

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3D Bioprinting Paves the Way for Fertility Preservation with VitroINK®

VitroINK® provided a xeno-free, structurally supportive bioink that enabled the precise 3D bioprinting of a functional testis model, facilitating cell viability, maturation, and in vitro spermatogenesis research. Fertility preservation following pediatric cancer therapy [...]

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Scaffold-Free 3D Adipose Tissue Organoids: Unlocking Obesity Research with VitroGel® 3D

VitroGel® 3D provided a scaffold-free yet supportive matrix that stabilized adipose organoids for imaging and analysis, enhancing the reliability of in vitro obesity research. Hydrogel:VitroGel® 3D (Cat. No: TWG001) Obesity and metabolic diseases [...]

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Advancing Borderline Ovarian Tumor Research: Precision Viability Assessment in Patient-Derived Organoid Models

Biomarker Cyto3D® Live-Dead Assay Kit (Cat. No: BM01) The study explores the application of tumor organoid technology in borderline ovarian tumors (BOT), a category of tumors with limited treatment options and resistance to [...]

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3D Organoids and Talniflumate: A Powerful Duo Against Pancreatic Cancer

Hydrogel: VitroGel® ORGANOID 3  (Cat. No: VHM04-3) Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers, with limited treatment options and poor patient survival rates. A major challenge in treating PDAC is [...]

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Revolutionizing Cancer Research: A Patient-Derived Platform to Model Tumor-Immune Interactions

BiomarkerCyto3D® Live-Dead Assay Kit (Cat. No: BM01) Cancer research has long been challenged by the complexity of tumor-immune interactions. Traditional 2D cell cultures and animal models often fail to capture the intricate tumor [...]

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Game Changer for Lymphoma Therapy Screening

VitroGel® RGD played a key role in preserving tumor architecture for effective drug testing in lymphoma research. Hydrogel:VitroGel® RGD (Cat. No: TWG003) VitroGel® played a The fight against cancer, particularly B-cell lymphomas, is [...]

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Prolonged Dexamethasone Delivery Using VitroGel® for Protection Against Cisplatin-Induced Ototoxicity

VitroGel® significantly enhanced the delivery of dexamethasone to the inner ear, providing controlled release and improved protection against cisplatin-induced hearing loss. Hydrogel: VitroGel® RGD (Cat. No: TWG003) VitroGel® played a crucial role in [...]

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Harnessing Patient-Derived Organoids to Decode Immunotherapy Responses in Colorectal Cancer

VitroGel® revolutionizes colorectal cancer research by creating a xeno-free platform for patient-derived organoids, enabling groundbreaking insights into tumor-immune interactions and immunotherapy responses. Hydrogel:VitroGel® MSC (Cat. No: VHM03) Other VitroGel® option:VitroGel® ORGANOID-3 (Cat. No: [...]

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VitroGel® ORGANOID Unlocks Precision High-Throughput Drug Screening Insights in Ovarian Cancer

VitroGel® ORGANOID empowered breakthrough single-cell pharmacotranscriptomic profiling by enabling robust, patient-specific cancer organoid models for drug discovery and resistance analysis. Hydrogel:VitroGel® ORGANOID-4 (Cat. No: VHM04-4) In this study, Dini et al. present a [...]

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VitroGel® ORGANOID Reveals Unique Pathway Shifts in HOSE Cells Exposed to Tumor Microenvironment

VitroGel® ORGANOID-4 deepened ovarian cancer research by allowing 3D spheroid formation and transcriptomic analysis, identifying key signaling STAT1 and GREM1 activation pathways. Institutions:University of Oulu; Institute for Molecular Medicine Finland; University of Helsinki; [...]

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News & Events

NEW PRODUCT: VitroGel® NEURON

TheWell Bioscience Unveils VitroGel® NEURON: A Breakthrough in 3D and 2D Neuron Culture TheWell Bioscience [...]

The International Society for Stem Cell Research (ISSCR) – 2025

We are excited to announce that TheWell Bioscience will be exhibiting at the ISSCR 2025 [...]

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