Category Archives: Application Notes

New hPSCs Expansion Method – Direct from Liquid Nitrogen to 3D Culture Using a Xeno-free Hydrogel – VitroGel® STEM

Culturing and maintaining human pluripotent stem cells (hPSCs) directly from cryopreservation using the xeno-free, hydrogel-based VitroGel® STEM Application Note TheWell Bioscience, North Brunswick, NJ 08902 Introduction Due to the advent of stem cell technologies, there has been an unprecedented rise in the use of human pluripotent stem cells (hPSCs) in biomedical research. Because of its […]

Advanced Skin Cell Models Using the VitroGel® System: 2D Coating and 3D Cell Culture with Human Dermal Fibroblasts

Application Note Bhavya Shah, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Despite recent progression, the need for reliable, repeatable in vitro three-dimensional (3D) tissue culture human skin models exist to further understand the mechanisms behind various pathologies related to the skin and/or dermal fibroblasts1-3. Such 3D models are increasingly important as two-dimensional (2D) […]

Advanced 3D Luminal Breast Cancer Model in VitroGel® System: Long-term 3D Cell Culture and Co-culture with Fibroblast Cells

Application Note Nana-Fatima Haruna, Bhavya Shah,John HuangTheWell Bioscience, North Brunswick, NJ 08902 Introduction There is an increasing need for the generation of data that accurately reflects the biological systems of patients 1-3. To achieve this goal, researchers need to study disease models in microenvironments that depict the complex human extracellular matrix and support the comprehensive […]

Long Term 3D Tumor Spheroid Culture in VitroGel Hydrogel Matrix

Application Note Nana-Fatima Haruna, Bhavya Shah, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Cancer is a very meticulous disease that involves heterogeneous solid tumors subjected to complex extracellular conditions. These conditions, such as hypoxia and extracellular compositions, contribute to the pathogenesis of the disease. The functional activity and gene expression patterns of the […]

3D Invasion of Glioblastoma Cells in VitroGel Hydrogel System

Application Note Nana-Fatima Haruna, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Glioblastoma is the most common primary tumor in the central nervous system. With an incidence of 3–5 out of 100,000 persons in Western countries, glioblastoma is one of the most deadly forms of malignant tumors, which has a median survival time of […]

Using the VitroGel Hydrogel System as a Versatile Platform for Invasion Assay

Application Note Nana-Fatima Haruna, Atsuo Ochi, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Invasion studies have been crucial in identifying key intracellular signaling pathways and membrane-bound and soluble enzymes that influence cellular movement through a three dimensional (3D) extracellular matrix (ECM)1,2. Such information has been used to investigate mechanisms governing the infiltration of […]

3D Printed Tumor Spheroids for Disease Modeling and Chemotherapeutic Drug Screening

Poster Presentation TheWell Bioscience Inc. Aragaw Gebeyehu1, Sunil Surapaneni1, John Huang2, Arindam Mondal1, Vivian Wang2, Nana-Fatima Haruna2, Peggy Arthur1, Shallu Kutlehria1, Nil Patel1, Mandip Sachdeva1 1College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL3230; 2TheWell Bioscience, Inc., North Brunswick NJ 08902 Abstract 3D bioprinting is intended for regenerative medicine and disease model development […]

Characterizing the Biophysical Properties of a Tunable Hydrogel System for 3D Cell Culture

Poster Presentation TheWell Bioscience Inc. Nana Fatima Haruna, Ziwen Vivian Wang, John Huang TheWell Bioscience, Inc., North Brunswick NJ 08902 Abstract The hydrogel-based 3D cell culture model more accurately depicts the microenvironment of cells in vivo than the traditional 2D cell culture system, which enables cells to grow, develop, operate, and produce feedback as if […]

3D Cell Culture of Glioblastoma Cell (U-87 MG) in VitroGel® Hydrogel System

Application Note TheWell Bioscience Inc. Introduction Glioblastoma is an aggressive type of cancer that occurs in the brain or spinal cord. Over the years, various models of glioblastoma have been developed to help understand the mechanism initiation and progression1,2. However, this has been complicated by the diverse and complex genetic and environmental factors that promote […]

3D Cell Culture of Bone Marrow Stroma Cells (OP9) on VitroGel® Hydrogel System

Application Note TheWell Bioscience Inc. Introduction Mesenchymal stem cells are a type of adult multipotent stem cell that gives rise to bone, cartilage, adipose, and connective tissue. Over the last two decades, they have become a wildly popular potential alternative to embryonic stem cells for regenerative medicine because of their broad plasticity, immunomodulatory, and anti-inflammatory […]