Category Archives: Application Notes

Comparing the Xeno-Free VitroGel to Animal-Based Matrigel for Xenograft Studies

Introduction A cell-derived xenograft (CDX) model is the gold standard for anti-cancer therapies. The response of xenografts obtained in immune-deficient animals in drug testing is comparable to that in clinical practice, which makes xenograft models robust research tools in oncology1. In addition, over the past decades, patient-derived xenograft (PDX) models have emerged for precision medicine […]

Xeno-free Organoid Generation Workflow for Stem Cell Spheroids Using VitroGel® STEM and VitroGel® ORGANOID Hydrogel System

Application Note TheWell Bioscience, North Brunswick, NJ 08902 Over the past decade, advances in stem cells and 3D cell culture technologies have reignited interest in organoid research. Organoids are derived from stem cells such as human pluripotent stem cells (hPSCs) or stem/progenitor cells and are composed of different cell types that are able to self-organize, […]

3D Spheroid Invasion Assay With the Xeno-free, Bio-Functional VitroGel® Hydrogel Matrix

Application Note Nana-Fatima Haruna, John HuangTheWell Bioscience, North Brunswick, NJ 08902 Introduction Cell migration and invasion play important roles in various physiological processes such as gastrulation, embryonic morphogenesis, development of the nervous system, and immune cell trafficking. Cells need to be able to change, interact with their environment, and reach their proper spatial orientation in […]

New hPSCs Expansion Method – Direct from Liquid Nitrogen to 3D Culture Using a Xeno-free Hydrogel – VitroGel® STEM

Culturing and maintaining human pluripotent stem cells (hPSCs) directly from cryopreservation using the xeno-free, hydrogel-based VitroGel® STEM Application Note TheWell Bioscience, North Brunswick, NJ 08902 Introduction Due to the advent of stem cell technologies, there has been an unprecedented rise in the use of human pluripotent stem cells (hPSCs) in biomedical research. Because of its […]

Advanced Skin Cell Models Using the VitroGel® System: 2D Coating and 3D Cell Culture with Human Dermal Fibroblasts

Application Note Bhavya Shah, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Despite recent progression, the need for reliable, repeatable in vitro three-dimensional (3D) tissue culture human skin models exist to further understand the mechanisms behind various pathologies related to the skin and/or dermal fibroblasts1-3. Such 3D models are increasingly important as two-dimensional (2D) […]

Advanced 3D Luminal Breast Cancer Model in VitroGel® System: Long-term 3D Cell Culture and Co-culture with Fibroblast Cells

Application Note Nana-Fatima Haruna, Bhavya Shah,John HuangTheWell Bioscience, North Brunswick, NJ 08902 Introduction There is an increasing need for the generation of data that accurately reflects the biological systems of patients 1-3. To achieve this goal, researchers need to study disease models in microenvironments that depict the complex human extracellular matrix and support the comprehensive […]

Long Term 3D Tumor Spheroid Culture in VitroGel Hydrogel Matrix

Application Note Nana-Fatima Haruna, Bhavya Shah, John HuangTheWell Bioscience, North Brunswick, NJ 08902 Introduction Cancer is a very meticulous disease that involves heterogeneous solid tumors subjected to complex extracellular conditions. These conditions, such as hypoxia and extracellular compositions, contribute to the pathogenesis of the disease. The functional activity and gene expression patterns of the tumor […]

3D Invasion of Glioblastoma Cells in VitroGel Hydrogel System

Application Note Nana-Fatima Haruna, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Glioblastoma is the most common primary tumor in the central nervous system. With an incidence of 3–5 out of 100,000 persons in Western countries, glioblastoma is one of the most deadly forms of malignant tumors, which has a median survival time of […]

Using the VitroGel Hydrogel System as a Versatile Platform for Invasion Assay

Application Note Nana-Fatima Haruna, Atsuo Ochi, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Invasion studies have been crucial in identifying key intracellular signaling pathways and membrane-bound and soluble enzymes that influence cellular movement through a three dimensional (3D) extracellular matrix (ECM)1,2. Such information has been used to investigate mechanisms governing the infiltration of […]

3D Printed Tumor Spheroids for Disease Modeling and Chemotherapeutic Drug Screening

Poster Presentation TheWell Bioscience Inc. Aragaw Gebeyehu1, Sunil Surapaneni1, John Huang2, Arindam Mondal1, Vivian Wang2, Nana-Fatima Haruna2, Peggy Arthur1, Shallu Kutlehria1, Nil Patel1, Mandip Sachdeva1 1College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL3230; 2TheWell Bioscience, Inc., North Brunswick NJ 08902 Abstract 3D bioprinting is intended for regenerative medicine and disease model development […]