VitroGel® IKVAV High Concentration

Laminin-derived functional peptide (IKVAV) modified – tunable, xeno-free hydrogel, high concentration ( 3 mL kit)

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VitroGel IKVAV High Concentration

VitroGel® IKVAV High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (IKVAV). IKVAV is the bioactive sequence located on the C-terminal end of the long arm of the α‐1 chain of laminin, which is actively involved in different biological activities such as neuronal progenitor cell differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth.  VitroGel IKVAV High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Mix & Match – 3D Cell Culture Your WAY!

Unique to VitroGel High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel.  VitroGel® IKVAV can be “mix & matched” with other versions of VitroGel such as VitroGel® RGD, VitroGel® YIGSR, VitroGel® MMP and VitroGel® COL to create the customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists can customize their 3D culture micro-environment for different applications.

Learn more about the “Mix & Match” features >

Specifications

ContentsVitroGel® IKVAV High Concentration, 3 mL
VitroGel® Dilution Solution, 50 mL
Hydrogel Formulation Xeno-free tunable hydrogel modified with IKVAV peptide.
UseSupport neuronal progenitor cells differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth
Mix & MatchCan be blended with other versions of VitroGel concentrated hydrogels to create a custom multi-functional matrix.
OperationRoom temperature
Hydrogel Strength10 to 4,000 Pa of G’ depending on dilution ratio.
Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations.
pHNeutral
ColorTransparent
Cell HarvestingVitroGel Organoid Recovery Solution
5-15 min cell recovery
InjectableInjectable hydrogel
StorageStore at 2-8°C. Ships at ambient temperature
Number of UsesDilution ratio:
1:2 = 225 uses at 50 µL per well
1:3 = 300 uses at 50 µL per well
1:5 = 450 uses at 50 µL per well

3D Cell Culture Process in 20 Minutes

VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.

Tunable Hydrogel Strength

Simply diluting the hydrogel controls the gel strength.

Protocols / Handbooks / Resources

Video Protocols & Demonstrations

VIDEO PROTOCOL TIP

3D Cell Culture
VitroGel High Concentration Hydrogels

VIDEO PROTOCOL TIP

2D Cell Coating
Method 1

VitroGel High Concentration Hydrogels

VIDEO PROTOCOL TIP

2D Cell Coating
Method 2

VitroGel High Concentration Hydrogels

VIDEO PROTOCOL TIP

Cell Recovery
Method 1

from 3D & 2D cultures

VIDEO PROTOCOL TIP

Cell Recovery
Method 2

from 3D & 2D  cultures

VIDEO PROTOCOL TIP

Injectable Hydrogel
VitroGel System

Data and References

Cell Type Behavior Reference Table – VitroGel IKVAV

Multiple uses of immobilized, conjugated IKVAV in different tissue and cell type. IKVAV has been used as an immobilized, adhesive substrata for multiple cell types to study many different cellular processes and behaviors in normal physiological and pathological contexts.

Cell TypeBehavior
Glioma LRM55Promoted cell attachment
Melanoma A2058Increased type IV collagenolytic activity
Melanoma K-1735Increased cell invasion
Melanoma SK-MEL-28Increased cell adhesion and proliferation
Prostate PC3Increased cell growth and invasion
Cell TypeBehavior
Rat skin fibroblastsIncreased cell adhesion
Cell TypeBehavior
Pulmonary fibroblasts LL29Increased cell adhesion
Cell TypeBehavior
Human neural stem cells Promoted cell viability and differentiation
Mouse neural progenitor celPromoted cell adhesion and differentiation
Mouse spiral ganglion neurons Promoted neurite outgrowth
Neural PC12Promoted neurite outgrowth
Rat cortical astrocytesIncreased cell adhesion
Rat neural stem cellsPromote cell attachment and differentiation
Cell TypeBehavior
Β-cells MIN6Increased insulin release and reduced apoptosis
Cell TypeBehavior
Human adipose derived stem cellsIncreased cell attachment
Human mesenchymal stem cellsIncreased cell viability
Human mesenchymal stem cellsPromoted neuronal differentiation
Cell TypeBehavior
Human umbilical vein endothelial cellsIncreased cell infiltration
Human outgrowth endothelial cells and bonePromoted angiogenesis
marrow mesenchymal stem cells
TISSUE/ORGAN TYPECELL TYPEREADY TO USE HIGH CONCENTRATIONBEHAVIOR
Beta Cell BL5 human beta cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3D,
VitroGel MMP
Enhance spheroids formation
Beta TC3 cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cellular interactions
Bone Bone marrow stromal cells (rat)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Osteogensic differentiation , Cell attachment and osteoblast differentiation,Cell proliferation, cell viability, and cellular networking.
Osteoblasts (rat) VitroGel MSC,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell attachment and spreading
Bone marrow stromal cells (bovine) VitroGel MSC,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell spreading and osteocalcin expression
BreastMammary gland MCF10AVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitrolGel RGD,
VitroGel COL,
VitroGel MMP
Spheroid formation, MMP activity in response to TGF-B1
Mammary epithelium (mouse)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell invasion and dissemination
Cancer/TumorHuman colorectal carcinoma HCT 116VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation, cell survival, and intercellular networking
Huaman colon carcinoma HCT-8 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Glioma U87-MG VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP,
VitroGel COL
Cell spreading and acting stress fiber assembly, cell proliferation, spreading, and migration, Cell migration dependent on mechancial force, Cell proliferation and cell matrix interaction
Gliobastoma SF 268VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitrolGel RGDCell proliferation and cell matrix interaction
Gliobastoma SF 295VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Glioblastoma SNB75VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Glioblastoma U-251 MGVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Prostate PC3VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel COL,
VitroGel IKVAV,
VitroGel RGD,
VitroGel MMP
Cell proliferation, reduced MMP release, invasion, migration, and spheroid metabolic activity.
Prostate LNCaPVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell attachment, proliferation, and prostate specific antigen release
Prostate CRPCVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and invasion
Prostate DU145VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and invasion
Melanoma B16F10VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel COL,
VitroGel YIGSR
Cell migration, invasion, MMP release, cell attachment and spreading
Breast MDA-MB-231VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP,
VitroGel 3D
Cell invasion, spreading, proliferation, division, migration, and cluster growth
Fibrosarcoma HT1080VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell infiltration, attachment
Breast T47DVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel COL,
VitroGel 3D,
VitroGel RGD,
VitroGel MMP
Force dependent tubule formation, cell cluster growth, spheroid formation and proliferation
Breast 4T1VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation
Breast CTCVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3D,
VitroGel RGD
Cell proliferation
Breast E0771VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation spheroid formation
Brest AU-565VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation cell matrix interactions
Epithelial ovarian OV-MZ-6 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDSpheroid formation and proliferation
Epithelial ovarian SKOV-3 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDSpheroid formation and proliferation
Glioma U373-MG VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell adhesion, invasion and migration
Rhabdomyosarcoma (human) VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel YIGSR
Cell attachment and spreading
Melanoma SK-MEL-28 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Cell adhesion and proliferation
Melanoma K-1735VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Cell invasion
Melanoma A2058VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Collagenolytic activity
Brainstem glioma DIPGVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell proliferation and survival
Hela Cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3D,
VitroGel RGD,
VitroGel MMP
Cell proliferation
Colorectal adenocarcinoma DLD-1 cellsVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Giloma LRM55VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel IKVAV,
VitroGel MMP
Cell attachment
Melanoma WM 239AVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell invasion
Melanoma Cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction
Insulinoma ins-1 (Rat)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Biphasic synovial sarcoma SYO-1VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation, cell matrix interation, and cell survival
Fuji Cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Chordoma Cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DCell proliferation
Bone OSA 1777VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDSpheroid and cluster formation
Glioma RuGliVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel COL Integrin dependent cell adhesion
Breast Cancer MCF-7 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP,
VitroGel 3D
Cell proliferation, intercellular connections, morphological changes, MMP expression, and angiogenesis
Liver carcinoma HepG2 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell viability, growth, drug resistance, proliferation, and cellular matrix interaction
Human pancreatic cancer PANC-1 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell proliferation and cellular interactions
Primary breast (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell invasion, migration, and dissemination
Ovarian carcinoma OVCAR-3 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP
Cell proliferation, cell matrix interactions
Ovarian OVCA429 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP,
VitroGel COL
MMP dependent cell invasion
Human Osteosarcoma KHOSVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel 3D
Cell proliferation and spheroids formation
Human Osteosarcoma U2OSVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel 3D
Cell proliferation and spheroids formation
Human fibroblast-like synoviocytes (FLS)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DCell proliferation and inflammatory responses
Human Liposarcoma 94T778VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DCell proliferation and spheroids formation
Human diffuse large B-cell lymphoma (DLBLC) SUDHL-10VitroGel Hydrogel MatrixCell viability, growth, drug resistance, proliferation, and cellular matrix interaction
Priess human lymphoblastoid cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DEnhance spheroids and cluster formation and promote cell viability.
CartilageChondrocytes (bovine) VitroGel MSC,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell viability and proliferation
Chondrocytes (human) VitroGel MSC,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell viability and proliferation
Connective TissueDermal Fibroblasts (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell viability and spreading
Fibroblasts NIH3T3VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Directional cell migration toward gradient and cell spreading dependent on substrata rigidity
Foreskin fibroblasts (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel YIGSR,
VitroGel MMP
Cell spreading, substrata degradation, and cell invasion
Skin fibroblasts (skin) VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel IKVAV
Cell adhesion
Epidermal keratinocytesVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell viability
Epithelial CellsMouse ovarian follicle cellsVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD3D cell culture using ES-hydrogel can enhance vitro follicle culture by considering the permeability and stiffness of the gel.
Human Nthy-ori 3-1 cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DEnhance spheroids and cluster formation and promote cell viability.
A549 cellsVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDEnhance cell proliferation and cell matrix interactions.
MCF-12AVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDEnhance cell proliferation and cell matrix interactions.
Immortalized bronchial epithelial cells HBEC-KRASVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel 3D
Cell proliferation
EyeCorneal endothelial B4G12VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit,
VitroGel Angiogenesis Assay
VitroGel RGD,
Vitrogel Angiogenesis Assay HC kit
Cell attachment and spreading
Retinal ganglion cells (xenopus)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Neurite outgrowth
Immune Cells CD8 + T cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DEnhance spheroids and cluster formation and promote cell viability.
KidneyHuman embryonic kidney HEK293 VitroGel Hydrogel Matrix,
VitroGel HEK293,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
3D spheroids formation
Madin-Darby Canine KidneyVitroGel Hydrogel Matrix,
VitroGel HEK293,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP
Epithelial cysts formation
Podocytes (human)VitroGel Hydrogel Matrix,
VitroGel HEK293,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Glomerular capillary formation
glomerular endothelial cells (human)VitroGel Hydrogel Matrix,
VitroGel HEK293,
VitroGel ORGANOID Kit,
VitroGel Angiogenesis Assay
VitroGel RGD,
Vitrogel Angiogenesis Assay HC kit
Glomerular capillary formation
Liver Hepatocytes (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Filopodia formation and synthesis of albumin and cell attachment
Hepatocytes (mouse, rat, swine)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell viability, spearding, Albumin secretion
LungAlveolar basal epithelial A549VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell attachment
Alveolar epithelial RLE-6TNVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell attachment and mesenchymal differentiation
Pulmonary fibroblasts LL2 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel IKVAV
Cell adhesion
HFL1 lung fibroblasts CCL153VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and spindle morphology
Lung cancer associated fibroblasts (human) VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDSubstrata contractility
Lung fibroblasts MCR-5VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
NGF-mediated substrata contraction
Muscle Myoblasts C2C12 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell proliferation, differentiation, attachment, myofibril formation, myotube formation, and integrin dependent cell adhesion
Skeletal myoblasts (mouse)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell attachment, proliferation, and myofibril formation
Myoblasts (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell adhesion, alignment along fiber, and myotube formation
Myoblasts C25Cl48VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell proliferation, differentiation and myotube formation
Neural Dorsal root ganglion (chick)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Neurite formation and force dependent neurite outgrowth
Neural PC12VitroGel Hydrogel Matrix
VitroGel RGD,
VitroGel IKVAV
Neurite outgrowth
Neural stem cell/ progenitor cell (rat)VitroGel STEM,
VitroGel Hydrogel Matrix
VitroGel RGD,
VitroGel IKVAV
Cell viability, attachment, and differentiation
Neural stem cell/ progenitor cell (human)VitroGel STEM,
VitroGel Hydrogel Matrix
VitroGel RGD,
VitroGel IKVAV,
VitroGel COL
Cell viability, attachment, and differentiation
Schwann cells (rat)VitroGel Hydrogel MatrixVitroGel RGDCell attachment and migration
Neural stem cell/ progenitor cell (mouse)VitroGel STEMVitroGel RGD,
VitroGel IKVAV
Cell adhesion and differentiation
Cortical astrocytes (rat)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel IKVAV
Cell adhesion
Spiral ganglion neurons (mouse)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel IKVAV
Neurite outgrowth
Motor neurons (human)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Force dependent neurite outgrowth
Forebrain neurons (human)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Force dependent neurite outgrowth
Cortical neurons (rat)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Neuronal viability and neurite outgrowth
Dorsal root ganglion (rat)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Neurite outgrowth
Red Blood CellsRed Blood CellsVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DEnhance Spheroids and cluster formation and promote cell viability
PancreasB-cells MIN6 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel IKVAV
Reduced apoptosis and increased insulin release
Stem Cells Mesenchymal stem cells (human)VitroGel MSCVitroGel RGD,
VitroGel COL,
VitroGel IKVAV,
VitroGel MMP
Cell viability, proliferation, differentiation, neuronal differntiation, neurite outgrowth, attachment, spreading, viability, and osteoblast differentiation
Mesenchymal stem cells (mouse)VitroGel MSCVitroGel RGD,
VitroGel MMP
Cell spreading and migration
Mesenchymal stem cells (rat)VitroGel MSCVitroGel RGDCell adhesion and spreading
Embryonic stem cells (mouse)VitroGel STEM,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel YIGSR
Endothelial cell differentiation, neuronal differentiation, and neurite outgrowth
Induced pluripotent stem cells (human)VitroGel STEM,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel YIGSR,
VitroGel IKVAV
Cell viability
Human stem cells from apical papilla SCAPVitroGel STEMVitroGel RGDCell viability
Hematopoietic Stem CellsVitroGel STEMCell viability
Adipose derived stem cells (human)VitroGel MSCVitroGel RGD,
VitroGel 3D,
VitroGel IKVAV
Cell viability, cell attachment
Vascular/cardiac Umbilical vein endothelial cells Vitrogel Angiogenesis Assay ,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
Vitrogel Angiogenesis Assay HC kitCell attachment, proliferation, migration, angiogenesis, gene expression changes, migratory cell infiltration, cell survival, and VEGF dependent migration
Neonatal cardiac (rat) Vitrogel Angiogenesis Assay,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
Vitrogel Angiogenesis Assay HC kitCell attachment, tissue regeneration, and attachment similar to laminin
Aortic smooth muscle cells VitroGel Hydrogel MatrixVitrogel Angiogenesis Assay HC kitCell attachment
Endothelial (human)VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCell differentiation
EndotheliocytesVitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCell migration
Microvascular endothelial cells (human)VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCell mobility
Aortic endothelial cells (bovine)VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitForce dependent cell spreading
Capillary endothelial cells (bovine) VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCapillary like network formation

Data


Figure 1. Rheological properties of VitroGel IKVAV with DMEM medium.
 A) The gel formation curve after mixing with DMEM medium. VitroGel IKVAV was diluted at 1:0,1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with DMEM at 4:1 (v/v) ratio; B) The gel strength after 24 hrs incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa)

Figure 2. 3D culture of glioblastoma cells (SNB 75) in VitroGel IKVAV.
Cells were cultured with 1:3 diluted VitroGel IKVAV according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration)
.

Mix & Match Hydrogel System – 3D Cell Culture Your Way
Mix VitroGel IKVAV with VitroGel MMP at different ratios

Figure 3. 3D culture of glioblastoma cells (SNB 75) in the mixture of VitroGel IKVAV and VitroGel MMP.
The mixing ratios of VitroGel IKVAV and VitroGel MMP are 3:1, 1:1 and 3:1 (V/V). Cells were cultured with a 1:3 diluted VitroGel mixture following the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration)
.

References/Publications

High Concentration Kit Type

VitroGel IKVAV + Dilution Solution TYPE 1, VitroGel IKVAV + Dilution Solution TYPE 2