VitroGel® IKVAV High Concentration
Laminin-derived functional peptide (IKVAV) modified – tunable, xeno-free hydrogel, high concentration ( 3 mL kit)
$429.00
VitroGel IKVAV High Concentration
VitroGel® IKVAV High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (IKVAV). IKVAV is the bioactive sequence located on the C-terminal end of the long arm of the α‐1 chain of laminin, which is actively involved in different biological activities such as neuronal progenitor cell differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth. VitroGel IKVAV High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.
Mix & Match – 3D Cell Culture Your WAY!
Unique to VitroGel High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel® IKVAV can be “mix & matched” with other versions of VitroGel such as VitroGel® RGD, VitroGel® YIGSR, VitroGel® MMP and VitroGel® COL to create the customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists can customize their 3D culture micro-environment for different applications.
Specifications
| Contents | VitroGel® IKVAV High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
| Hydrogel Formulation | Xeno-free tunable hydrogel modified with IKVAV peptide. HIGH CONCENTRATION |
| Use | Support neuronal progenitor cells differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth |
| Mix & Match | Can be blended with other versions of VitroGel to create a multi-functional hydrogel |
| Operation | Room temperature |
| Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
| pH | Neutral |
| Color | Transparent |
| Cell Harvesting | 20 min cell recovery using VitroGel Cell Recovery Solution |
| Injectable | Injectable hydrogel |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Number of Uses | Dilution ratio: 1:2 = 225 uses at 50 µL per well 1:3 = 300 uses at 50 µL per well 1:5 = 450 uses at 50 µL per well |
3D Cell Culture Process in 20 Minutes
VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.
Tunable Hydrogel Strength
Simply diluting the hydrogel controls the gel strength.
Handbooks and Resources
Video Protocols & Demonstrations
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
Data and References
Cell Type Behavior Reference Table for VitroGel IKVAV
Multiple uses of immobilized, conjugated IKVAV in different tissue and cell type. IKVAV has been used as an immobilized, adhesive substrata for multiple cell types to study many different cellular processes and behaviors in normal physiological and pathological contexts.
| Cell Type | Behavior |
|---|---|
| Glioma LRM55 | Promoted cell attachment |
| Melanoma A2058 | Increased type IV collagenolytic activity |
| Melanoma K-1735 | Increased cell invasion |
| Melanoma SK-MEL-28 | Increased cell adhesion and proliferation |
| Prostate PC3 | Increased cell growth and invasion |
| Cell Type | Behavior |
|---|---|
| Rat skin fibroblasts | Increased cell adhesion |
| Cell Type | Behavior |
|---|---|
| Pulmonary fibroblasts LL29 | Increased cell adhesion |
| Cell Type | Behavior |
|---|---|
| Human neural stem cells | Promoted cell viability and differentiation |
| Mouse neural progenitor cel | Promoted cell adhesion and differentiation |
| Mouse spiral ganglion neurons | Promoted neurite outgrowth |
| Neural PC12 | Promoted neurite outgrowth |
| Rat cortical astrocytes | Increased cell adhesion |
| Rat neural stem cells | Promote cell attachment and differentiation |
| Cell Type | Behavior |
|---|---|
| Β-cells MIN6 | Increased insulin release and reduced apoptosis |
| Cell Type | Behavior |
|---|---|
| Human adipose derived stem cells | Increased cell attachment |
| Human mesenchymal stem cells | Increased cell viability |
| Human mesenchymal stem cells | Promoted neuronal differentiation |
| Cell Type | Behavior |
|---|---|
| Human umbilical vein endothelial cells | Increased cell infiltration |
| Human outgrowth endothelial cells and bone | Promoted angiogenesis |
| marrow mesenchymal stem cells |
| Tissue/Organ type | Cell Type | Relate product | Behavior |
|---|---|---|---|
| Beta cell | BL5 human beta cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Beta TC3 cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cellular interations | |
| Bone | Bone marrow stromal cells (rat) | VitroGel Hydrogel Matrix, VitroGel RGD | Osteogenesic differentiation |
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell viability, and cellular networking | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment and osteoblast differentiation | ||
| Bone marrow mesenchymal stem cells (human) | VitroGel Hydrogel Matrix, VitroGel COL | Chondrogenic/osteogenic differentiation | |
| VitroGel Hydrogel Matrix, VitroGel IKVAV | Angiogenesis | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading, proliferation, and collagen II production | ||
| Bone marrow mesenchymal stem cells (goat) | VitroGel Hydrogel Matrix, VitroGel RGD | Osteogenesic differentiation | |
| Osteoblasts (rat) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment and spreading | |
| Bone marrow stromal cells (bovine) | VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading and osteocalcin expression | |
| Breast | Mammary gland MCF10A | VitroGel Hydrogel Matrix, VitroGel MMP | MMP activity in response to TGF-ß1 |
| Mammary epithelium (mouse) | VitroGel Hydrogel Matrix, VitroGel COL | Cell invasion and dissemination | |
| Cancer/tumor | Human colorectal carcinoma HCT 116 | VitroGel Hydrogel Matrix, VitroGel RGD | cell proliferation, cell survival, and intercelluar networking |
| Huaman colon carcinoma HCT-8 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioma U87-MG | VitroGel Hydrogel Matrix, VitroGel RGD | Cell spreading and actin stress fiber assembly | |
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell migration dependent on mechanical force | ||
| VitroGel Hydrogel Matrix, VitroGel MMP | cell proliferation, spreading, and migration | ||
| Primary glioblastom U87 | VitroGel Hydrogel Matrix, VitroGel RGD | cell proliferation and cellular interations | |
| Glioblastoma SF 268 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioblastoma SF 295 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioblastoma SNB75 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioblastoma U-251 MG | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Prostate PC3 | VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and reduced MMP release | |
| VitroGel Hydrogel Matrix, VitroGel IKVAV | cell proliferation and invasion | ||
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and invasion | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell invasion, migration, and spheroid metabolic activity | ||
| Prostate LNCaP | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and prostate specific antigen release | ||
| Prostate CRPC | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferatin and invasion | |
| Prostate DU145 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and invasion | |
| Melanoma B16F10 | VitroGel Hydrogel Matrix, VitroGel COL | Cell migration, invasion, and MMP release | |
| VitroGel Hydrogel Matrix, VitroGel YIGSR | Cell attachment and spreading | ||
| Breast MDA-MB-231 | VitroGel Hydrogel Matrix, VitroGel MMP | Cell invasion | |
| VitroGel Hydrogel Matrix | Cell spreading | ||
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, division, migration, and invasion | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading and cluster growth | ||
| Fibrosarcoma HT1080 | VitroGel Hydrogel Matrix, VitroGel COL | Cell infiltration | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment | ||
| Breast T47D | VitroGel Hydrogel Matrix, VitroGel COL | Force dependent tubule formation | |
| VitroGel Hydrogel Matrix | Cell cluster growth | ||
| VitroGel Hydrogel Matrix, VitroGel 3D | Spheroid formation and proliferation | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell cluster growth | ||
| Breast 4T1 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation | |
| Breast CTC | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Breast E0771 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, spheroid formation | |
| Breast AU-565 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell matrix interations | |
| Epithelial ovarian OV-MZ-6 | VitroGel Hydrogel Matrix, VitroGel RGD | Spheroid formation and proliferation | |
| Epithelial ovarian SKOV-3 | VitroGel Hydrogel Matrix, VitroGel RGD | Spheroid formation and proliferation | |
| Glioma U373-MG | VitroGel Hydrogel Matrix, VitroGel RGD | Cell adhesion and migration | |
| Rhabdomyosarcoma (human) | VitroGel Hydrogel Matrix, VitroGel YIGSR | Cell attachment and spreading | |
| Melanoma SK-MEL-28 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell adhesion and proliferation | |
| Melanoma K-1735 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell invasion | |
| Melanoma A2058 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Collagenolytic activity | |
| Brainstem glioma DIPG | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation and survival | |
| Hela Cells | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Colorectal adenocarcinoma DLD-1 cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioma LRM55 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell attachment | |
| Melanoma WM239A | VitroGel Hydrogel Matrix, VitroGel MMP | Cell invasion | |
| Melanoma Cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Insulinoma ins-1 (Rat) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| HEK 293 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Biphasic synovial sarcoma SYO-1 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell matirx interaction, and cell survival | |
| Fuji Cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Chordoma Cells | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Bone OSA 1777 | VitroGel Hydrogel Matrix, VitroGel RGD | spheroid and cluster formation | |
| Glioma RuGli | VitroGel Hydrogel Matrix, VitroGel COL | Integrin dependent cell adhesion | |
| Breast Cancer MCF-7 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, intercellular connections | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation, morphological changes, MMP expression, and angiogenesis | ||
| Liver carcinoma HepG2 | VitroGel Hydrogel Matrix, VitroGel COL | Cell viability, growth, and drug resistance | |
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | ||
| Human pancreatic cancer PANC-1 | VitroGel Hydrogel Matrix, VitroGel RGD | cell proliferation and cellular interations | |
| Primary breast (human) | VitroGel Hydrogel Matrix, VitroGel COL | Cell invasion, migration, and dissemination | |
| Ovarian carcinoma OVCAR-3 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell matrix interations | |
| Ovarian OVCA429 | VitroGel Hydrogel Matrix, VitroGel COL | MMP dependent cell invasion | |
| Human osteosarcoma KHOS | VitroGel Hydrogel Matrix, VitroGel 3D | cell proliferation and spheroids formation | |
| Human osteosarcoma U2OS | VitroGel Hydrogel Matrix, VitroGel 3D | cell proliferation and spheroids formation | |
| Priess human lymphoblastoid cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| Cartilage | Chondrocytes (bovine) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability and proliferation |
| Chondrocytes (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability and proliferation | |
| Connective tissue | Dermal fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability and spreading |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell viability | ||
| Fibroblasts NIH3T3 | VitroGel Hydrogel Matrix, VitroGel RGD | Directional cell migration toward gradient | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading dependent on substrata rigidity | ||
| Foreskin fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell spreading | |
| VitroGel Hydrogel Matrix, VitroGel YIGSR | Cell spreading | ||
| VitroGel Hydrogel Matrix, VitroGel MMP | Substrata degradation and cell invasion | ||
| Skin fibroblasts (skin) | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell adhesion | |
| Epidermal keratinocytes | VitroGel Hydrogel Matrix, VitroGel COL | Cell viability | |
| Epithelial Cells | Mouse ovarian follicle cells | VitroGel Hydrogel Matrix, VitroGel RGD | 3D cell culture using ES-hydrogel can enhance vitro follicle culture by considering the permeability and stiffness of the gel. |
| Human Nthy-ori 3-1 cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| A549 cells | VitroGel Hydrogel Matrix, VitroGel RGD | Enhance cell proliferation and cell matrix interactions. | |
| MCF-12A | VitroGel Hydrogel Matrix, VitroGel RGD | Enhance cell proliferation and cell matrix interactions. | |
| Immortalized bronchial epithelial cells HBEC-KRAS | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Eye | Corneal endothelial B4G12 | VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment and spreading |
| Retinal ganglion cells (xenopus) | VitroGel Hydrogel Matrix, VitroGel COL | Neurite outgrowth | |
| Immune Cells | CD8 + T cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Kidney | Human embryonic kidney HEK293 | VitroGel Hydrogel Matrix, VitroGel RGD | 3D spheroids formation |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and cluster growth | ||
| Madin-Darby Canine Kidney | VitroGel Hydrogel Matrix, VitroGel RGD | Epithelial cysts formation | |
| podocytes (human) | VitroGel Hydrogel Matrix, VitroGel COL | Glomerular capillary formation | |
| glomerular endothelial cells (human) | VitroGel Hydrogel Matrix, VitroGel COL | Glomerular capillary formation | |
| Liver | Hepatocytes (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Filopodia formation and synthesis of albumin |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment | ||
| Hepatocytes (mouse) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability | |
| Hepatocytes (rat) | VitroGel Hydrogel Matrix, VitroGel COL | Albumin secretion | |
| Hepatocytes (swine) | VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading and albumin section | |
| Lung | Alveolar basal epithelial A549 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment |
| Alveolar epithelial RLE-6TN | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment and mesenchymal differentiation | |
| Pulmonary fibroblasts LL2 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell adhesion | |
| HFL1 lung fibroblasts CCL153 | VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and spindle morphology | |
| Lung cancer associated fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel COL | Substrata contractility | |
| Lung fibroblasts MCR-5 | VitroGel Hydrogel Matrix, VitroGel COL | NGF-mediated substrata contraction | |
| Muscle | Myoblasts C2C12 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell Proliferation and differentiation |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment, proliferation, and myofibril formation | ||
| VitroGel Hydrogel Matrix | Myotube formation | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Integrin dependent cell adhesion | ||
| Skeletal myoblasts (mouse) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment, proliferation, and myofibril formation | |
| Myoblasts (human) | VitroGel Hydrogel Matrix, VitroGel COL | Cell adhesion, alignment along fiber, and myotube formation | |
| Myoblasts C25Cl48 | VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation, differentiation and myotube formation | |
| Neural | Dorsal root ganglion (chick) | VitroGel RGD | Neurite formation and outgrowth |
| VitroGel COL | Force dependent neurite outgrowth | ||
| Neural PC12 | VitroGel COL | Neurite outgrowth | |
| VitroGel IKVAV | Neurite outgrowth | ||
| Neural stem cell/progenitor cell (rat) | VitroGel YIGSR | Cell viability | |
| VitroGel IKVAV | Cell attachment and differentiation | ||
| Neural stem cell/progenitor cell (human) | VitroGel IKVAV | Cell viability and differentiation | |
| VitroGel LDP1 | Cell viability and differentiation | ||
| VitroGel LDP1 | Cell viability | ||
| VitroGel COL | Cell attachment | ||
| Schwann cells (rat) | VitroGel YIGSR | Cell attachment and migration | |
| Neural stem cell/progenitor cell (mouse) | VitroGel IKVAV | Cell adhesion and differentiation | |
| Cortical astrocytes (rat) | VitroGel IKVAV | Cell adhesion | |
| Spiral ganglion neurons (mouse) | VitroGel IKVAV | Neurite outgrowth | |
| Motor neurons (human) | VitroGel COL | Force dependent neurite outgrowth | |
| Forebrain neurons (human) | VitroGel COL | Force dependent neurite outgrowth | |
| Cortical neurons (rat) | VitroGel COL | Neuronal viability and neurite outgrowth | |
| Dorsal root ganglion (rat) | VitroGel COL | Neurite outgrowth | |
| Red Blood Cells | Red Blood cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Pancreas | B-cells MIN6 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Reduced apoptosis and increased insulin release |
| Stem cells | Mesenchymal stem cells (human) | VitroGel RGD | Cell viability |
| VitroGel RGD | Cell Proliferation and differentiation | ||
| VitroGel COL | Cell proliferation | ||
| VitroGel IKVAV | Neuronal differentiation | ||
| VitroGel MMP | Neuronal differentiation and neurite outgrowth | ||
| VitroGel COL | Cell attachment, spreading, viability, and osteoblast differentiation | ||
| Mesenchymal stem cells (mouse) | VitroGel RGD | Cell spreading and migration | |
| VitroGel MMP | Cell spreading and migration | ||
| Mesenchymal stem cells (rat) | VitroGel RGD | Cell adhesion and spreading | |
| Embryonic stem cells (mouse) | VitroGel RGD | Endothelial cell differentiation | |
| VitroGel COL | Neuronal differentiation and neurite outgrowth | ||
| VitroGel YIGSR | Neuronal differentiation | ||
| Induced pluripotent stem cells (human) | VitroGel YIGSR | Cell viability | |
| VitroGel IKVAV | Cell viability | ||
| VitroGel LDP1 | Cell viability | ||
| Human Ipsc | VitroGel RGD | Cell proliferation, and cell matrix interactions | |
| Human stem cells from apical papilla SCAP | VitroGel 3D | Cell viability | |
| Adipose derived stem cells (human) | VitroGel IKVAV | Cell attachment | |
| Vascular/cardiac | Umbilical vein endothelial cells (human) | VitroGel RGD | Cell attachment, proliferation, migration, and angiogenesis |
| VitroGel YIGSR | Upregulation in gene expression | ||
| VitroGel IKVAV | Migratory cell infiltration | ||
| VitroGel MMP | Cell attachment, migration, and survival | ||
| VitroGel COL | Cell attachment, spreading, and VEGF dependent migration | ||
| Neonatal cardiac (rat) | VitroGel RGD | Cell attachment and tissue regeneration | |
| VitroGel YIGSR | Cell attachment similar to laminin | ||
| Aortic smooth muscle cells (human) | VitroGel RGD | Cell attachment | |
| Endothelial (human) | VitroGel YIGSR | Cell differentiation | |
| Endotheliocytes | VitroGel YIGSR | Cell migration | |
| Microvascular endothelial cells (human) | VitroGel YIGSR | Cell mobility | |
| Aortic endothelial cells (bovine) | VitroGel COL | Force dependent cell spreading | |
| Capillary endothelial cells (bovine) | VitroGel COL | Capillary like network formation |
Data
Figure 1. Rheological properties of VitroGel IKVAV with DMEM medium.
A) The gel formation curve after mixing with DMEM medium. VitroGel IKVAV was diluted at 1:0,1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with DMEM at 4:1 (v/v) ratio; B) The gel strength after 24 hrs incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa)
Figure 2. 3D culture of glioblastoma cells (SNB 75) in VitroGel IKVAV.
Cells were cultured with 1:3 diluted VitroGel IKVAV according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).
Mix & Match Hydrogel System – 3D Cell Culture Your Way
Mix VitroGel IKVAV with VitroGel MMP at different ratios
Figure 3. 3D culture of glioblastoma cells (SNB 75) in the mixture of VitroGel IKVAV and VitroGel MMP.
The mixing ratios of VitroGel IKVAV and VitroGel MMP are 3:1, 1:1 and 3:1 (V/V). Cells were cultured with a 1:3 diluted VitroGel mixture following the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).
References/Publications
- References to all VitroGel hydrogels >
- Powell K. Adding depth to cell culture. Science, 356(6333), 96–98. https://doi.org/10.1126/science.356.6333.96
| Size | 3 mL Kit |
|---|
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