VitroGel® HEK293
ready-to-use, xeno-free (animal origin-free) hydrogel system for 3D culture of HEK293 cells
VitroGel® HEK293
VitroGel® HEK293 is a xeno-free (animal origin-free) functional hydrogel system developed to support three-dimensional (3D) cultures of human embryonic kidney 293 (HEK293) cells.
- Simple 5-minute protocol
- High-quality 3D spheroids
- Easily scale up with flasks and bioreactors
- Easy cell harvesting protocol (Enzyme-free, No trypsin)
VitroGel® HEK293 is a unique system that easily supports the rapid 3D growth of HEK293 cells. Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D static suspension cultures. Both our standard 3D cell culture protocol and the 3D static suspension culture protocols can be used for rapid cell expansion. The high-quality 3D spheroids enhance the cell performance for protein expression and downstream applications. HEK293 cells cultured in VitroGel® HEK293 can easily be scaled up for large-scale bioreactors or spin flasks. By using the VitroGel Cell Recovery Solution, cell harvesting after 3D culture is simple and effective.
“Just add cells” 5 min protocol 3D static suspension culture
VitroGel® HEK293 is ready-to-use. Just mix with your cells. There is no cross-linking agent and no need to adjust the hydrogel concentration.
“Just add cells” 20 min 3D cell culture process
VitroGel® HEK293 is ready to use. Just mix it with your cells. There is no cross-linking agent, and there is no need to adjust the hydrogel concentration.
Specifications
| Formulation | Xeno-free, functional hydrogel |
| Use | 3D cell culture, 2D hydrogel coating, hydrogel-cell bead formation |
| Operation | Ready-to-use at room temperature |
| Biocompatibility | Biocompatible, safe for animal studies |
| Injection | Injectable hydrogel for in vivo studies and lab automation |
| Cell Harvesting | VitroGel Organoid Recovery Solution 5-15 min cell recovery |
| pH | Neutral |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Sizes | 10 mL and 2 mL |
| Number of Uses | (10 mL) 300 uses at 50 µL per well (2 mL) 60 uses at 50 µL per well |
Recommended Product
Recover cells from the hydrogel within 15 minutes with high cell viability. Non-enzymatic system.
VitroGel® Organoid Recovery Solution
Protocols / Handbooks / Resources
Product Documentations
Product Data Sheet
Frequently Asked Questions
Material Safety Data Sheet (MSDS)
Video Protocols & Demonstrations
Data and References
3D Cell Model
Figure 1. 3D static suspension culture of HEK293 cells in VitroGel® HEK293.
Cells were mixed with VitroGel® HEK293 at (2:1 gel/cells v/v ratio) and then mixed with cell culture medium at 1:3 ratio (cell-gel mixture: culture medium at 1:3 v/v). HEK293 cells form cell spheroids in the hydrogel. The images show the growth and expansion from day 0 to day 7. The enlarged images show the cell spheroids after 7 days of culture. The cell proliferation was tested using the Cell Counting Kit-8 (CCK-8) assay. The curve shows the increase in cell number in a 14-day culture.
Figure 2. Cell viability of HEK293 cells in 3D suspension culture in VitroGel HEK293.
3D static suspension cultures of HEK293 cells in VitroGel® HEK293 were stained with the Cyto3D® Live/Dead Assay Kit (Cat # BM01). The live cells are shown in green, and the dead cells are shown in orange. The images show cells cultured in VitroGel® HEK293 hydrogel performing with high cell viability.
Figure 3. 3D static suspension culture of HEK293 cells with high cell density seeding (24 hours).
For protein production purposes, cell suspension can be prepared at high cell density (107 cells/mL) to reach the peak of protein expression within 48 to 72 hours. The images show the fast formation of cell spheroids with over 100 µm size in diameter 24 hours after the cell was seeded in VitroGel® HEK293 hydrogel for static suspension culture.
Figure 4. Subculture of HEK293 cell spheroids with VitroGel.
The fibroblast-like mouse bone marrow stromal cells (OP9-GFP) were cultured in 3D. The cell spheroid culture with VitroGel® HEK293 can be collected by centrifuging (100 x g, 3 min). Please check the cell harvesting protocol of VitroGel® Organoid Recovery Solution (CAT# MS04-100 for details). The collected cell spheroid can be dissociated into single cells by trypsin. After that, the cells can be resuspended with culture medium and mixed with VitroGel® HEK293 for subculture. The images show the formation of cell spheroids from day 0 to day 5 after subculture.
References/Publications
| Size | 2 mL, 10 mL |
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