VitroGel® Cell Invasion Assay Kit

A simple and easy animal-free replacement for your protocol.
This kit includes the VitroGel® Hydrogel Matrix with VitroPrime™ Cell Culture Inserts, 8 µm, PET

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VitroGel® Cell Invasion Assay Kit (Ready-To-Use)

Your gateway for easy-to-use and consistent in-depth cell invasion studies.

Go beyond your traditional animal-based ECM for invasion studies with our xeno-free VitroGel® hydrogels and VitroPrime™ Cell Culture Inserts.

Cell invasion, a vital process in various biological contexts, can be pivotal in embryonic development, immunosurveillance, and wound healing while also playing a concerning role in cancer metastasis. Traditional in vitro invasion assays have relied on animal-based extracellular matrices, which come with challenges like undefined components, batch-to-batch variability, and cumbersome temperature-sensitive protocols, thus leading to inconsistent and inaccurate studies.

The VitroGel® Cell Invasion Assay Kit is powered by VitroGel® hydrogel (versatile, xeno-free, bio-functional hydrogels that closely mimic the physiological extracellular matrix) and coupled with our premium quality VitroPrime™ Cell Culture Inserts, allowing more accurate and consistent invasion and migration studies than animal-based ECM.

The VitroGel® Cell Invasion Assay Kit (Ready-To-Use) in this page (includes our VitroGel® Hydrogel Matrix) is an optimal choice to simply replace the animal-based ECM for most traditional cell mobility studies. The nature of this synthetic system also allows researchers to precisely design the experiment by adjusting supplements and desired factors within the hydrogel matrix.

We also provide tunable VitroGel® High-Concentration Cell Invasion Assay Kit, which offers unmatched flexibility, allowing researchers to fully customize the hydrogel matrix—adjusting mechanical strength, binding ligands, and ECM components. No other product enables such a deep exploration of cell mobility and invasion studies, providing researchers with the precision and control needed to push the boundaries of their experiments like never before. Learn about our tunable VitroGel® High-Concentration Cell Invasion Assay Kits >

Specifications

UseCell Invasion, cell migration, co-culture, barrier model
InsertsVitroPrime™ Cell Culture Inserts
8 µm, PET, Transparent, Sterile
Kit ContentsVitroGel® Hydrogel Matrix + VitroPrime™ Cell Culture Inserts
FormulationXeno-free, functional hydrogel
pHNeutral
StorageStore hydrogel at 2-8°C. Ships at ambient temperature

Cell invasion culture process in 30 minutes.

Work confidently at room temperature. No ice bucket required. VitroGel® Cell Invasion Assay Kit is ready-to-use. There is no cross-linking agent required.
Learn how gelation works.

Type of studies capable with VitroGel® Cell Invasion Assay Kit (Ready-To-Use)

Comparison of VitroGel® versus traditional animal-based ECM for cell invasion studies

Ready-To-Use VitroGel®-Based Cell Invasion Assay

Traditional assay with animal-based ECM

Operation temperature

Room temperature

2-8 °C

Set up time

30 mins

2 hours +

Control compounds of outer well

Consistent results

Control key compounds in hydrogel

Control mechanical strength of hydrogel

Study functional ligands of hydrogel

Control hydrogel degradation

High-throughput / Lab automation

Protocols and Resources

Product Data Sheet

Videos & Webinars

VitroGel® Cell Invasion Assay Kit Protocol

Advanced Cell Mobility Studies Using the Xeno-free VitroGel®-Based Cell Invasion Assay Kits

Application Notes

 Research Highlights

Data and References

Chemoattraction from outer well

Adding chemoattractants into the outer well to study cell mobility with ready-to-use VitroGel® Hydrogel Matrix system.

Invasion of U87-MG Glioblastoma cells towards an FBS gradient

  • Cell Invasion Assay Kit: VitroGel® Cell Invasion Assay (Ready-to-use, Cat # IA-VHM01-4P)
  • Insert: VitroGel® Hydrogel Matrix
  • Outer well: No serum v.s. 20% FBS
  • Cell incubation time: 48 hrs

Invasion of U87-MG glioblastoma cells through VitroGel® Hydrogel Matrix caused by a serum gradient.
A.
Schematic representation demonstrating the invasion assay cell culture set-up. B. U-87 MG cell invasion was visualized by performing crystal violet staining followed by light microscopy. The images show the membrane inserts from control group (No FBS) and 20% FBS conditions. Images were obtained with a Zeiss microscope at a 10X magnification. C. Fold change of U87-MG cell invasion between control and 20% FBS groups. The control group was normalized to 1. The asterisk (*) stands for p<0.05.

Effects of cytokine within the hydrogel matrix on cell mobility

The ready-to-use VitroGel® Hydrogel Matrix allows researchers to manipulate the chemokines, cytokines, growth factors, etc., within the hydrogel matrix to understand their effects on cell mobility.

Take Advantage of the Synthetic Nature and Well Characterized Composition of VitroGel® And Assay Growth Factor and Drugs within the Hydrogel

  • Cell Invasion Assay Kit: VitroGel® Cell Invasion Assay (Ready-to-use, Cat # IA-VHM01-4P)
  • Insert: VitroGel® Hydrogel Matrix with TGF-β1 v.s. without TGF-β1
  • Outer well: No serum v.s. 20% FBS
  • Cell Incubation time: 24 hrs

TGF-β1 inside of VitroGel® hydrogel matrix induces invasion of U87-MG glioblastoma cells.
A.
Visual representation of invasion assay setup. B. Light microscopy images demonstrating cell invasion in the different groups after crystal violet staining. Images were obtained with a Zeiss microscope at a 10X magnification. C. Mean of U87-MG cell invasion for each of the experimental conditions.

References/Publications

View more publications on VItroGel® Products
Assay Kit Configuration

1 mL + (12) VitroPrime™ Cell Culture Inserts, 4 mL + (48) VitroPrime™ Cell Culture Inserts