VitroGel® 3D High Concentration

tunable, xeno-free hydrogel, high concentration (3 mL kit)

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VitroGel 3D High Concentration

VitroGel® 3D High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system that allows the maximum flexibility to manipulate the 3D cell culture environment for different needs. VitroGel 3D High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa. The hydrogel’s tunability gives researchers the ability to create an optimized environment for cell growth. The VitroGel 3D High Concentration hydrogel matrix structure is good for cell spheroid formation, suspension cells, or cells requiring low cell-matrix interactions.

VitroGel High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications

ContentsVitroGel® 3D High Concentration, 3 mL
VitroGel® Dilution Solution, 50 mL
Hydrogel FormulationXeno-free tunable hydrogel, pure and unmodified.
UseGood for cell spheroid formation, suspension cells or cells require low cell-matrix interactions
Mix & MatchCan be blended with other versions of VitroGel concentrated hydrogels to create a custom multi-functional matrix.
OperationRoom temperature
Hydrogel Strength10 to 4,000 Pa of G’ depending on dilution ratio.
Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations.
pHNeutral
ColorTransparent
Cell Harvesting20 min cell recovery using VitroGel Cell Recovery Solution
InjectableInjectable hydrogel
StorageStore at 2-8°C. Ships at ambient temperature
Number of UsesDilution ratio:
1:2 = 225 uses at 50 µL per well
1:3 = 300 uses at 50 µL per well
1:5 = 450 uses at 50 µL per well

3D Cell Culture Process in 20 Minutes

VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.

Tunable Hydrogel Strength

Simply diluting the hydrogel controls the gel strength

Handbooks and Resources

Video Protocols & Demonstrations

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

Application Notes

APPLICATION NOTE

APPLICATION NOTE

APPLICATION NOTE

Data and References

Cell Type Behavior Reference Table – VitroGel 3D

Studies performed using VitroGel 3D in different tissue and cell types.

Cancer/Tumor
Cell TypeBehavior
Brainstem glioma DIPGCell proliferation and survival
Breast CTCCell proliferation
Breast T47DSpheroid formation and proliferation
Chordoma CellsCell proliferation
Hela CellsCell proliferation
Human osteosarcoma KHOSCell proliferation and spheroid formation
Human osteosarcoma U2OSCell proliferation and spheroid formation
Epithelial Cells
Cell TypeBehavior
Human NTHY-ORI 3-1 CellsEnhance spheroids and cluster formation and promote cell viability
Immune Cells
Cell TypeBehavior
BL5 human beta cellsEnhance spheroids and cluster formation and promote cell viability
CD8 + T cellsEnhance spheroids and cluster formation and promote cell viability
Priess human lymphoblastoid cellsEnhance spheroids and cluster formation and promote cell viability
Red Blood Cells
Cell TypeBehavior
Red Blood CellsEnhance spheroids and cluster formation and promote cell viability
Stem Cells
Cell TypeBehavior
Human stem cells from apical papilla SCAPEnhance cell viability
All Cell Type Behaviors for All VitroGel Products
TISSUE/ORGAN TYPECELL TYPEREADY TO USE HIGH CONCENTRATIONBEHAVIOR
Beta Cell BL5 human beta cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3D,
VitroGel MMP
Enhance spheroids formation
Beta TC3 cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cellular interactions
Bone Bone marrow stromal cells (rat)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Osteogensic differentiation , Cell attachment and osteoblast differentiation,Cell proliferation, cell viability, and cellular networking.
Osteoblasts (rat) VitroGel MSC,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell attachment and spreading
Bone marrow stromal cells (bovine) VitroGel MSC,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell spreading and osteocalcin expression
BreastMammary gland MCF10AVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitrolGel RGD,
VitroGel COL,
VitroGel MMP
Spheroid formation, MMP activity in response to TGF-B1
Mammary epithelium (mouse)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell invasion and dissemination
Cancer/TumorHuman colorectal carcinoma HCT 116VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation, cell survival, and intercellular networking
Huaman colon carcinoma HCT-8 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Glioma U87-MG VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP,
VitroGel COL
Cell spreading and acting stress fiber assembly, cell proliferation, spreading, and migration, Cell migration dependent on mechancial force, Cell proliferation and cell matrix interaction
Gliobastoma SF 268VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitrolGel RGDCell proliferation and cell matrix interaction
Gliobastoma SF 295VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Glioblastoma SNB75VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Glioblastoma U-251 MGVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Prostate PC3VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel COL,
VitroGel IKVAV,
VitroGel RGD,
VitroGel MMP
Cell proliferation, reduced MMP release, invasion, migration, and spheroid metabolic activity.
Prostate LNCaPVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell attachment, proliferation, and prostate specific antigen release
Prostate CRPCVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and invasion
Prostate DU145VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and invasion
Melanoma B16F10VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel COL,
VitroGel YIGSR
Cell migration, invasion, MMP release, cell attachment and spreading
Breast MDA-MB-231VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP,
VitroGel 3D
Cell invasion, spreading, proliferation, division, migration, and cluster growth
Fibrosarcoma HT1080VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell infiltration, attachment
Breast T47DVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel COL,
VitroGel 3D,
VitroGel RGD,
VitroGel MMP
Force dependent tubule formation, cell cluster growth, spheroid formation and proliferation
Breast 4T1VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation
Breast CTCVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3D,
VitroGel RGD
Cell proliferation
Breast E0771VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation spheroid formation
Brest AU-565VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation cell matrix interactions
Epithelial ovarian OV-MZ-6 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDSpheroid formation and proliferation
Epithelial ovarian SKOV-3 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDSpheroid formation and proliferation
Glioma U373-MG VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell adhesion, invasion and migration
Rhabdomyosarcoma (human) VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel YIGSR
Cell attachment and spreading
Melanoma SK-MEL-28 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Cell adhesion and proliferation
Melanoma K-1735VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Cell invasion
Melanoma A2058VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Collagenolytic activity
Brainstem glioma DIPGVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell proliferation and survival
Hela Cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3D,
VitroGel RGD,
VitroGel MMP
Cell proliferation
Colorectal adenocarcinoma DLD-1 cellsVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Giloma LRM55VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel IKVAV,
VitroGel MMP
Cell attachment
Melanoma WM 239AVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell invasion
Melanoma Cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction
Insulinoma ins-1 (Rat)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Biphasic synovial sarcoma SYO-1VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation, cell matrix interation, and cell survival
Fuji Cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and cell matrix interaction
Chordoma Cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DCell proliferation
Bone OSA 1777VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDSpheroid and cluster formation
Glioma RuGliVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel COL Integrin dependent cell adhesion
Breast Cancer MCF-7 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP,
VitroGel 3D
Cell proliferation, intercellular connections, morphological changes, MMP expression, and angiogenesis
Liver carcinoma HepG2 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell viability, growth, drug resistance, proliferation, and cellular matrix interaction
Human pancreatic cancer PANC-1 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell proliferation and cellular interactions
Primary breast (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell invasion, migration, and dissemination
Ovarian carcinoma OVCAR-3 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP
Cell proliferation, cell matrix interactions
Ovarian OVCA429 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP,
VitroGel COL
MMP dependent cell invasion
Human Osteosarcoma KHOSVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel 3D
Cell proliferation and spheroids formation
Human Osteosarcoma U2OSVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel 3D
Cell proliferation and spheroids formation
Human fibroblast-like synoviocytes (FLS)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DCell proliferation and inflammatory responses
Human Liposarcoma 94T778VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DCell proliferation and spheroids formation
Human diffuse large B-cell lymphoma (DLBLC) SUDHL-10VitroGel Hydrogel MatrixCell viability, growth, drug resistance, proliferation, and cellular matrix interaction
Priess human lymphoblastoid cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DEnhance spheroids and cluster formation and promote cell viability.
CartilageChondrocytes (bovine) VitroGel MSC,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell viability and proliferation
Chondrocytes (human) VitroGel MSC,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell viability and proliferation
Connective TissueDermal Fibroblasts (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell viability and spreading
Fibroblasts NIH3T3VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Directional cell migration toward gradient and cell spreading dependent on substrata rigidity
Foreskin fibroblasts (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel YIGSR,
VitroGel MMP
Cell spreading, substrata degradation, and cell invasion
Skin fibroblasts (skin) VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel IKVAV
Cell adhesion
Epidermal keratinocytesVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell viability
Epithelial CellsMouse ovarian follicle cellsVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD3D cell culture using ES-hydrogel can enhance vitro follicle culture by considering the permeability and stiffness of the gel.
Human Nthy-ori 3-1 cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DEnhance spheroids and cluster formation and promote cell viability.
A549 cellsVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDEnhance cell proliferation and cell matrix interactions.
MCF-12AVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDEnhance cell proliferation and cell matrix interactions.
Immortalized bronchial epithelial cells HBEC-KRASVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel 3D
Cell proliferation
EyeCorneal endothelial B4G12VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit,
VitroGel Angiogenesis Assay
VitroGel RGD,
Vitrogel Angiogenesis Assay HC kit
Cell attachment and spreading
Retinal ganglion cells (xenopus)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Neurite outgrowth
Immune Cells CD8 + T cells VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DEnhance spheroids and cluster formation and promote cell viability.
KidneyHuman embryonic kidney HEK293 VitroGel Hydrogel Matrix,
VitroGel HEK293,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
3D spheroids formation
Madin-Darby Canine KidneyVitroGel Hydrogel Matrix,
VitroGel HEK293,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel MMP
Epithelial cysts formation
Podocytes (human)VitroGel Hydrogel Matrix,
VitroGel HEK293,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Glomerular capillary formation
glomerular endothelial cells (human)VitroGel Hydrogel Matrix,
VitroGel HEK293,
VitroGel ORGANOID Kit,
VitroGel Angiogenesis Assay
VitroGel RGD,
Vitrogel Angiogenesis Assay HC kit
Glomerular capillary formation
Liver Hepatocytes (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Filopodia formation and synthesis of albumin and cell attachment
Hepatocytes (mouse, rat, swine)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel MMP
Cell viability, spearding, Albumin secretion
LungAlveolar basal epithelial A549VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell attachment
Alveolar epithelial RLE-6TNVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell attachment and mesenchymal differentiation
Pulmonary fibroblasts LL2 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel IKVAV
Cell adhesion
HFL1 lung fibroblasts CCL153VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell proliferation and spindle morphology
Lung cancer associated fibroblasts (human) VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDSubstrata contractility
Lung fibroblasts MCR-5VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
NGF-mediated substrata contraction
Muscle Myoblasts C2C12 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell proliferation, differentiation, attachment, myofibril formation, myotube formation, and integrin dependent cell adhesion
Skeletal myoblasts (mouse)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGDCell attachment, proliferation, and myofibril formation
Myoblasts (human)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell adhesion, alignment along fiber, and myotube formation
Myoblasts C25Cl48VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Cell proliferation, differentiation and myotube formation
Neural Dorsal root ganglion (chick)VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL
Neurite formation and force dependent neurite outgrowth
Neural PC12VitroGel Hydrogel Matrix
VitroGel RGD,
VitroGel IKVAV
Neurite outgrowth
Neural stem cell/ progenitor cell (rat)VitroGel STEM,
VitroGel Hydrogel Matrix
VitroGel RGD,
VitroGel IKVAV
Cell viability, attachment, and differentiation
Neural stem cell/ progenitor cell (human)VitroGel STEM,
VitroGel Hydrogel Matrix
VitroGel RGD,
VitroGel IKVAV,
VitroGel COL
Cell viability, attachment, and differentiation
Schwann cells (rat)VitroGel Hydrogel MatrixVitroGel RGDCell attachment and migration
Neural stem cell/ progenitor cell (mouse)VitroGel STEMVitroGel RGD,
VitroGel IKVAV
Cell adhesion and differentiation
Cortical astrocytes (rat)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel IKVAV
Cell adhesion
Spiral ganglion neurons (mouse)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel IKVAV
Neurite outgrowth
Motor neurons (human)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Force dependent neurite outgrowth
Forebrain neurons (human)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Force dependent neurite outgrowth
Cortical neurons (rat)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Neuronal viability and neurite outgrowth
Dorsal root ganglion (rat)VitroGel Hydrogel MatrixVitroGel RGD,
VitroGel COL,
VitroGel IKVAV
Neurite outgrowth
Red Blood CellsRed Blood CellsVitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel 3DEnhance Spheroids and cluster formation and promote cell viability
PancreasB-cells MIN6 VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel IKVAV
Reduced apoptosis and increased insulin release
Stem Cells Mesenchymal stem cells (human)VitroGel MSCVitroGel RGD,
VitroGel COL,
VitroGel IKVAV,
VitroGel MMP
Cell viability, proliferation, differentiation, neuronal differntiation, neurite outgrowth, attachment, spreading, viability, and osteoblast differentiation
Mesenchymal stem cells (mouse)VitroGel MSCVitroGel RGD,
VitroGel MMP
Cell spreading and migration
Mesenchymal stem cells (rat)VitroGel MSCVitroGel RGDCell adhesion and spreading
Embryonic stem cells (mouse)VitroGel STEM,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel COL,
VitroGel YIGSR
Endothelial cell differentiation, neuronal differentiation, and neurite outgrowth
Induced pluripotent stem cells (human)VitroGel STEM,
VitroGel ORGANOID Kit
VitroGel RGD,
VitroGel YIGSR,
VitroGel IKVAV
Cell viability
Human stem cells from apical papilla SCAPVitroGel STEMVitroGel RGDCell viability
Hematopoietic Stem CellsVitroGel STEMCell viability
Adipose derived stem cells (human)VitroGel MSCVitroGel RGD,
VitroGel 3D,
VitroGel IKVAV
Cell viability, cell attachment
Vascular/cardiac Umbilical vein endothelial cells Vitrogel Angiogenesis Assay ,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
Vitrogel Angiogenesis Assay HC kitCell attachment, proliferation, migration, angiogenesis, gene expression changes, migratory cell infiltration, cell survival, and VEGF dependent migration
Neonatal cardiac (rat) Vitrogel Angiogenesis Assay,
VitroGel Hydrogel Matrix,
VitroGel ORGANOID Kit
Vitrogel Angiogenesis Assay HC kitCell attachment, tissue regeneration, and attachment similar to laminin
Aortic smooth muscle cells VitroGel Hydrogel MatrixVitrogel Angiogenesis Assay HC kitCell attachment
Endothelial (human)VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCell differentiation
EndotheliocytesVitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCell migration
Microvascular endothelial cells (human)VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCell mobility
Aortic endothelial cells (bovine)VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitForce dependent cell spreading
Capillary endothelial cells (bovine) VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCapillary like network formation

Data

Figure 1. Beta Lox 5 (BL5) cells 3D culture in VitroGel 3D system.
A.
BL5 cells culture on the surface of regular tissue culture treated well plate (control); B. Normal human islets grew in suspension culture (comparison); C. 3D culture of BL5 cells in VitroGel 3D at Day 1; D. 3D culture of BL5 cells in VitroGel 3D at Day 7. Under 3D culture of VitroGel 3D, BL5 cells form islet-like structures very similar to normal human islets. The hydrogel is prepared at 1:3 dilution. The images were taken at 10X magnification.

Figure 2. CD8+ T cells 3D culture in VitroGel 3D system.
CD8+ T cells culture grew in suspension culture (control); B. 3D culture of CD8+ T cells in VitroGel 3D at Day 7.  CD8+ T cells are vibrant in 3D culture conditions of VitroGel 3D. The cells can easily move within the unmodified hydrogel matrix. The hydrogel is prepared at 1:3 dilution. The images were taken at 10X magnification.

2D Coating Applications

Figure 3. Human colon cancer cells (HCT 116) cells cultured on top of VitroGel 3D hydrogel.
A thick hydrogel coating plate has been prepared by mixing VitroGel 3D with PBS at 1:1 ratio. A 300 µL mixture has been added to a well of a 24-well plate and stabilization at room temperature for 20 minutes before adding cells on top of the hydrogel. Cell spheroids form on the top of the hydrogel. Cells seeded at 2.5-10×105 cells/mL.

Figure 4. Comparison of long-term neuronal culture seeded onto thick hydrogel mats.
Cells are stained with Beta-III-Tubulin (green) cytoskeleton marker and their nuclei are counter-stained with DAPI (blue). Cells spread out and form neural-like networks as early as day 3 post-differentiation, with comparable efficacy between VitroGel 3D and Matrigel, based on cell survival, culture spreading, and morphological analysis reached between days 7 and 9. On Matrigel mats, cell culture health and viability drop off sharply once day 9 has passed, with most cells detaching and neurites retracting by day 14 and the vast majority of cells gone by day 21. If grown onto VitroGel 3D mats, differentiated B35 neurons have a tendency to self-organize into 3D clusters very early on (Day 7), assuming a mixed 2D/3D cell culture for the first two weeks of the time course. By Day 21, these cells have migrated into self-assembled 3D clusters, embedded into the thick hydrogel matrix, with very few cells between the clusters, but without any significant cell death.

Figure 5. Human Lymphoblastoid Priess cells cultured on top of VitroGel 3D hydrogel.
A.
Priess cells grown in suspension (control); B. Priess cells grown on top of VitroGel 3D at day 7. A hydrogel substance can be prepared with different stiffness by adjusting the dilution of VitroGel 3D from 1:1 to 1:3 ratio. Cells seeded on the top of the hydrogel form cell spheroids form on the top of the hydrogel. The hydrogel provides a soft substance for cells to attach and grow.

References/Publications

High Concentration Kit Type

VitroGel 3D + Dilution Solution TYPE 1, VitroGel 3D + Dilution Solution TYPE 2