VitroGel® ORGANOID

ready-to-use, xeno-free (animal origin-free) hydrogel system for organoid culture

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Overview

  • Xeno-free hydrogel for organoid formation
  • Supports a wide range of organoids from stem cells, tissues, co-culture and PDX resources
  • Scalable protocol for lab automation
  • Easy 20-minute cell harvesting (enzyme-free)

VitroGel® ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.

The VitroGel ORGANOID Discovery Kit (Cat# VHM04-K) includes all four types of organoid hydrogels (VitroGel ORGANOID-1, VitroGel ORGANOID-2, VitroGel ORGANOID-3, VitroGel ORGANOID-4) which were formulated with various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions.

VitroGel ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.

The organoid hydrogels can work in conjunction with VitroGel STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel STEM to VitroGel ORGANOID hydrogels for organoid differentiation. See Xeno-free 3D Organoid Workflow BKey growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution.  VitroGel ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.

Specifications

FormulationXeno-free, functional hydrogel
UseOrganoid culture
OperationReady-to-use at room temperature
BiocompatibilityBiocompatible, safe for animal studies
InjectionInjectable hydrogel for in vivo studies and lab automation
Cell HarvestingEasy cell recovery using VitroGel Cell Recovery Solution
pHNeutral
StorageStore at 2-8°C. Ships at ambient temperature
SizesSingle Vial: 10 mL
Discovery Kit: (2 mL) each of ORGANOID-1, ORGANOID-2, ORGANOID-3, ORGANOID-4
Number of Uses(10 mL) 300 uses at 50 µL/test
(2 mL) 60 uses at 50 µL/test

3D organoid cell culture process in 20 min – “Just add cells”

VitroGel ORGANOID is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.

Recommended Product

Harvest organoids from the hydrogel in 20 minutes with high cell viability. Enzyme-free system.

VitroGel Cell Recovery Solution

Handbooks and Resources

Video Protocols & Demonstrations

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

VitroGel Organoid Methods vs Natural ECM Methods

VitroGel ORGANOID system can culture organoids in variety of methods:

  • 2D hydrogel coating
  • 3D cell culture encapsulation
  • Hydrogel-Cell droplet (A unique method only to VitroGel ORGANOID)

Review all the following VitroGel ORGANOID protocol methods compared to the natural ECM methods and choose the one that best fits your project

Method 1
2D Hydrogel Coating

Add cells on top of hydrogel for optimal interactions among cells, hydrogel, and medium.

Organoid culture on 2D hydrogel workflow

Method 2
3D Cell Culture Encapsulation

Encapsulate cells within the hydrogel for excellent mechanical support and the cell-matrix interactions.

3D Organoid culture with VItroGel workflow

Method 3
Hydrogel-Cell Droplet

Create a VitroGel-Cell droplet. This is a unique culture method only to the VitroGel system for generating great cell-matrix interactions and excellent medium penetration for organoid formation and scale-up.

Organoid hydrogel-droplet culture method workflow

Data and References

Xeno-free 3D Organoid Workflow A: 
Using VitroGel ORGNAOID for organoid culture and maturation

Figure 1. Mouse intestinal organoid culture on VitroGel ORGANOID and Matrigel.
Small organoids recovered from liquid nitrogen were directly seeded with VitroGel and Matrigel, respectively. Images show the growth of mouse intestinal organoid from day 0 to day 14.

 

Xeno-free 3D Organoid Workflow B:
Start from iPSC spheroids for stem cell differentiation and organoid formation.
(Diagram below shows culturing human intestinal organoid from stem cell spheroids)

Figure 2. Culture human intestinal organoid from stem cell spheroids.
Human iPSCs recovered from liquid nitrogen were seeded with VitroGel STEM for static suspension culture. The high-quality stem cell spheroids formed within 3-5 days with full pluripotent properties (showing the positive markers of SSEA4, OCT4, SOX2, and TRA-1-60). The spheroids were harvested by centrifuging (100 x g, 3 min) and resuspended with VitroGel STEM in endoderm differentiation medium for 3 days. The endoderm cell spheroids were then harvested by centrifuging (100 x g, 3 min) and resuspended with VitroGel STEM in mid/hindgut differentiation medium for 3-4 days. The mid/hindguts were collected by centrifuging (100 x g, 3 min) and characterized with CDX2 and E-Cadherin. Resuspended the mid/hindgut with organoid formation medium and mix with VitroGel ORGANOID (follow the protocol of VitroGel ORGANOID) for organoid formation and long-term maturating culture.

Organoid 2D Hydrogel Coating Method

Figure 3. Organoids from breast cancer PDX cultured on VitroGel ORGANOID.
Cells were cultured on top of 2D hydrogel coating surface for 7 days.

 

Figure 4. Cerebral organoid culture with VitroGel ORGANOID and Matrigel comparison.

References/Publications


Matrigel is a trademark of Corning Incorporated.

Organoid Version

KIT, ORGANOID-1, ORGANOID-2, ORGANOID-3, ORGANOID-4