VitroPrime™ Spread-Attach Plate
50 packs/case
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VitroPrime™ Spread-Attach Cell Culture Plates
Particularly optimized for xeno-free biofunctional VitroGel systems, the combination of VitroGel and the VitroPrime™ Spread-Attach plates can unlock the full automation potential of hydrogel-based 3D cell culture.
Specifications
| Material | Polystyrene (GPPS) |
| Packaging | 50 plates/case |
| Well Plate Type | 6-well, 12-well, 24-well, 48-well, 96-well |
| Cell Growth Area (cm2) | 9.5 cm2 (6-well) 3.9 cm2 (12-well) 1.9 cm2 (24-well) 0.7 cm2 (48-well) 0.3 cm2 (96-well) |
| Treated Surface | Yes |
| Sterile | Yes |
Resources
Product Documentations
Data and References
Evaluating Gel Spreading and Cell Attachment – VitroPrime Spread-Attach 12-Well v. Standard Plate
We meticulously compared two 25-stitched z-planes capturing OP9 cells in the VitroGel Hydrogel Matrix within a 12-well plate—Standard Plate #1 and VitroPrime™ Spread-Attach Plate 12-Well. The images were compiled by selecting the z-plane with the 2D projection on each plate and stitched to evaluate cell attachment and the flatness of the hydrogel surface. Notably, VitroPrime™ Spread-Attach 12-Well plate demonstrated superior performance, ensuring flat hydrogel surface for even gel spreading and cell attachment.
Figure 1. A comparison of two 25-stitched z-planes within a well of a 12-well plate
A. Standard plate #1 shows uneven hydrogel surface by showing attach cells in different focus plane. B. VitroPrime™ Spread-Attach 12-Well plate. shows flat hydrogel surface by presenting homogeous cell spreading and attachment on the same plane.
Comparison of Hydrogel Adherance – VitroPrimeTM Spread-Attach v. Others
We investigated the effectiveness of VitroPrime Spread-Attach and three other plasticswares in mitigating the hydrogel floating issue using VitroGel ORGANOID-3 (VHM04-3) hydrogel.
The method involves combining hydrogel and basal medium in a 2:1 ratio, followed by adding 300 uL of VitroGel ORGANOID-3 hydrogel mixture to each well of a 24-well plate. The hydrogel is incubated for 20 minutes, after which 300 uL of cover medium is added on top. The plates are then incubated at 37°C to assess the ability of VitroPrime plasticware to prevent hydrogel floating.
Figure 2. Comparison of hydrogel adherence on four different quality 24-well plates in 48 hours.
A. Standard Plate #1 demonstrates hydrogel adherence in 21 out of 24 wells. B. Standard Plate #2 exhibits hydrogel adherence in 22 out of 24 wells.
C. Premium Plate #1 showcases hydrogel adherence in 23 out of 24 wells. D. VitroPrime™ Spread-Attach 24-Well plate exhibit flawless hydrogel adherence in all 24 wells.
| Plate Size | 6-well, 12-well, 24-well, 48-well, 96-well |
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