The VitroINK® Mixing Kit was designed to provide a robust mixing of the bioink and cells. Cells can be prepared with VitroINK for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. To prepare the bioprinter cartridge, the VitroINK and cell suspension are placed in the mixer and is dispensed through the connector and mixing head. Wait 10-20 minutes for the mixture to stabilized and the cartridge is ready for printing. There is no UV, no temperature/pH curing, or chemical cross-linking for the VitroINK system. Adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.