VitroGel® COL High Concentration
collagen-mimetic functional hydrogel – tunable, xeno-free (3 mL)
$429.00
VitroGel COL High Concentration
VitroGel COL High Concentration is a tunable, xeno-free hydrogel system that mimics the functions of native collagen. The hydrogel can promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo, which shows great potential for tissue engineering and regeneration medicine application. VitroGel COL High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.
Mix & Match – 3D Cell Culture Your WAY!
Unique to VitroGel High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel® COL can be “mix & matched” with other versions of VitroGel such as VitroGel® RGD, VitroGel® IKVAV, VitroGel® YIGSR, and VitroGel® MMP to create a customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists can customize their 3D culture micro-environment for different applications.
Specifications
| Contents | VitroGel® COL High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
| Hydrogel Formulation | Xeno-free tunable collagen-mimetic functional hydrogel. HIGH CONCENTRATION |
| Use | Support integrin binding to promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo |
| Mix & Match | Can be blended with other versions of VitroGel to create a multi-functional hydrogel |
| Operation | Room temperature |
| Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
| pH | Neutral |
| Color | Transparent |
| Cell Harvesting | 20 min cell recovery using VitroGel Cell Recovery Solution |
| Injectable | Injectable hydrogel |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Number of Uses | Dilution ratio: 1:2 = 225 uses at 50 µL per well 1:3 = 300 uses at 50 µL per well 1:5 = 450 uses at 50 µL per well |
3D Cell Culture Process in 20 Minutes
VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.
Tunable Hydrogel Strength
Simply diluting the hydrogel controls the gel strength.
Handbooks and Resources
Video Protocols & Demonstrations
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
Data and References
Cell Type Behavior Reference Table for VitroGel COL
Bone
| Cell Type | Behavior |
|---|---|
| Bovine bone marrow stromal cells | Increased cell spreading and osteocalcin expression |
| Human bone | Increased cell spreading, proliferation, and collagen II production |
| marrow mesenchymal stem cells | |
| Rat bone marrow stromal cells | Increased cell adhesion and osteoblast differentiation |
| Human bone marrow-derived mesenchymal stem cells | Promoted calcium deposition and chondrogenic/ |
| osteogenic differentiation | |
| Mouse bone marrow stromal cells | Supports osteogenesis |
Breast
| Cell Type | Behavior |
|---|---|
| Mouse mammary epithelium | Promoted transient cell invasion and dissemination |
Cancer/Tumor
| Cell Type | Behavior |
|---|---|
| Breast MDA-MB-231 | Increased cell cluster size and spreading |
| Breast T47D | Increased cell cluster size |
| Breast T47D | Promoted force dependent tubule formation |
| Breast MCF-7 | Increased cell proliferation, morphological changes, MMP expression, and angiogenesis |
| Co-culture of liver carcinoma HepG2 and stromal fibroblasts 3T3-J2 | Increased cell viability, growth, and drug resistance |
| Fibrosarcoma HT1080 | Support cell infiltration and growth |
| Fibrosarcoma HT1080 | Promoted integrin dependent cell adhesion |
| Fibrosarcoma HT1080 | Promoted cell adhesion |
| Glioma RuGli | Promoted integrin dependent cell adhesion |
| Glioma U87-MG | Cell migration dependent on mechanical force |
| Prostate PC3 | Increased cell invasion, migration, and spheroid metabolic activity |
| Human primary breast | Promoted cell invasion, migration, and dissemination |
| Melanoma B16F10 | Increased cell migration, invasion, and MMP release |
| Ovarian OVCA429 | MMP dependent cell invasion |
| Prostate LNCaP | Supported cell proliferation and increased prostate-specific antigen release |
| Prostate PC3 | Supported cell proliferation and reduced MMP release |
Connect Tissues
| Cell Type | Behavior |
|---|---|
| Co-culture human dermal fibroblasts and epidermal keratinocytes | Promoted cell viability |
| Fibroblast NIH3T3 | Increased cell spreading on rigid |
Eye
| Cell Type | Behavior |
|---|---|
| Corneal endothelial B4G12 | Increased cell attachment and spreading |
| Xenopus retinal ganglion cells | Promoted neurite outgrowth |
Liver
| Cell Type | Behavior |
|---|---|
| Human Hep3B | Promoted cell attachment |
| Rat hepatocytes | Promote albumin secretion |
| Swine hepatocytes | Promoted cell spreading and albumin section |
Lung
| Cell Type | Behavior |
|---|---|
| Lung fibroblasts HFL1 (CCL153) | Promoted cell proliferation and spindle morphology |
| Human lung cancer associated fibroblasts | Increased smooth muscle actin and substrata contractility |
| Lung fibroblasts MCR-5 | Promoted NGF-mediated substrata contraction |
Muscle
| Cell Type | Behavior |
|---|---|
| Human myoblasts | Promoted cell adhesion, alignment along fiber, and myotube formation |
| Mouse myoblast C2C12 | Promote integrin dependent cell adhesion |
| Myoblasts C2C12 | Promoted formation of myotubes and myotendinous |
| Myoblasts C2C12 | Promote cell proliferation, differentiation, and myotube formation |
| Myoblasts C25Cl48 | Promote cell proliferation, differentiation, and myotube formation |
Neural
| Cell Type | Behavior |
|---|---|
| Human neural stem/progenitor cells | Promoted cell attachment |
| Chick dorsal root ganglion | *Increased neurite length on soft |
| Chick dorsal root ganglion | *Increased neurite outgrowth toward soft |
| Human motor neurons | *Increased neurite length on rigid |
| Human forebrain neurons | *Increased neurite length on soft |
| Neural PC12 | Increased neurite length |
| Rat cortical neurons | Increased neuronal viability and neurite length |
| Rat dorsal root ganglion | Promoted axon outgrowth |
| Rat dorsal root ganglion | Promoted neurite outgrowth |
| Rat spinal cord section | Promoted neurite outgrowth |
Stem Cells
| Cell Type | Behavior |
|---|---|
| Human mesenchymal stem cells | Promoted cell adhesion, spreading, viability, and osteoblast differentiation |
| Human mesenchymal stem cells | Promoted chondrogenic differentiation |
| Human mesenchymal stem cells | Increased cell migration, proliferation, and osteogenic differentiation |
| Human mesenchymal stem cells | Promoted cell attachment and tenogenic differentiation |
| Human mesenchymal stem cells | Promoted cell proliferation |
| Mouse embryonic stem cells | Supported neuronal differentiation and neurite outgrowth |
Vascular
| Cell Type | Behavior |
|---|---|
| Bovine aortic endothelial cells | Promoted cell spreading along fiber |
| Bovine aortic endothelial cells | Increased cell spreading on rigid |
| Bovine capillary endothelial cells | Formation of capillary like networks |
| Human umbilical vein endothelial cells | Increased VEGF dependent vascularization |
| Human umbilical vein endothelial cells | Promoted cell adhesion, spreading and supported increased VEGF dependent migration |
View All Cell Type Behaviors for All VitroGel Products
| Tissue/Organ type | Cell Type | Relate product | Behavior |
|---|---|---|---|
| Beta cell | BL5 human beta cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Beta TC3 cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cellular interations | |
| Bone | Bone marrow stromal cells (rat) | VitroGel Hydrogel Matrix, VitroGel RGD | Osteogenesic differentiation |
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell viability, and cellular networking | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment and osteoblast differentiation | ||
| Bone marrow mesenchymal stem cells (human) | VitroGel Hydrogel Matrix, VitroGel COL | Chondrogenic/osteogenic differentiation | |
| VitroGel Hydrogel Matrix, VitroGel IKVAV | Angiogenesis | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading, proliferation, and collagen II production | ||
| Bone marrow mesenchymal stem cells (goat) | VitroGel Hydrogel Matrix, VitroGel RGD | Osteogenesic differentiation | |
| Osteoblasts (rat) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment and spreading | |
| Bone marrow stromal cells (bovine) | VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading and osteocalcin expression | |
| Breast | Mammary gland MCF10A | VitroGel Hydrogel Matrix, VitroGel MMP | MMP activity in response to TGF-ß1 |
| Mammary epithelium (mouse) | VitroGel Hydrogel Matrix, VitroGel COL | Cell invasion and dissemination | |
| Cancer/tumor | Human colorectal carcinoma HCT 116 | VitroGel Hydrogel Matrix, VitroGel RGD | cell proliferation, cell survival, and intercelluar networking |
| Huaman colon carcinoma HCT-8 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioma U87-MG | VitroGel Hydrogel Matrix, VitroGel RGD | Cell spreading and actin stress fiber assembly | |
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell migration dependent on mechanical force | ||
| VitroGel Hydrogel Matrix, VitroGel MMP | cell proliferation, spreading, and migration | ||
| Primary glioblastom U87 | VitroGel Hydrogel Matrix, VitroGel RGD | cell proliferation and cellular interations | |
| Glioblastoma SF 268 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioblastoma SF 295 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioblastoma SNB75 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioblastoma U-251 MG | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Prostate PC3 | VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and reduced MMP release | |
| VitroGel Hydrogel Matrix, VitroGel IKVAV | cell proliferation and invasion | ||
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and invasion | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell invasion, migration, and spheroid metabolic activity | ||
| Prostate LNCaP | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and prostate specific antigen release | ||
| Prostate CRPC | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferatin and invasion | |
| Prostate DU145 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and invasion | |
| Melanoma B16F10 | VitroGel Hydrogel Matrix, VitroGel COL | Cell migration, invasion, and MMP release | |
| VitroGel Hydrogel Matrix, VitroGel YIGSR | Cell attachment and spreading | ||
| Breast MDA-MB-231 | VitroGel Hydrogel Matrix, VitroGel MMP | Cell invasion | |
| VitroGel Hydrogel Matrix | Cell spreading | ||
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, division, migration, and invasion | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading and cluster growth | ||
| Fibrosarcoma HT1080 | VitroGel Hydrogel Matrix, VitroGel COL | Cell infiltration | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment | ||
| Breast T47D | VitroGel Hydrogel Matrix, VitroGel COL | Force dependent tubule formation | |
| VitroGel Hydrogel Matrix | Cell cluster growth | ||
| VitroGel Hydrogel Matrix, VitroGel 3D | Spheroid formation and proliferation | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell cluster growth | ||
| Breast 4T1 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation | |
| Breast CTC | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Breast E0771 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, spheroid formation | |
| Breast AU-565 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell matrix interations | |
| Epithelial ovarian OV-MZ-6 | VitroGel Hydrogel Matrix, VitroGel RGD | Spheroid formation and proliferation | |
| Epithelial ovarian SKOV-3 | VitroGel Hydrogel Matrix, VitroGel RGD | Spheroid formation and proliferation | |
| Glioma U373-MG | VitroGel Hydrogel Matrix, VitroGel RGD | Cell adhesion and migration | |
| Rhabdomyosarcoma (human) | VitroGel Hydrogel Matrix, VitroGel YIGSR | Cell attachment and spreading | |
| Melanoma SK-MEL-28 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell adhesion and proliferation | |
| Melanoma K-1735 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell invasion | |
| Melanoma A2058 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Collagenolytic activity | |
| Brainstem glioma DIPG | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation and survival | |
| Hela Cells | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Colorectal adenocarcinoma DLD-1 cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioma LRM55 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell attachment | |
| Melanoma WM239A | VitroGel Hydrogel Matrix, VitroGel MMP | Cell invasion | |
| Melanoma Cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Insulinoma ins-1 (Rat) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| HEK 293 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Biphasic synovial sarcoma SYO-1 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell matirx interaction, and cell survival | |
| Fuji Cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Chordoma Cells | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Bone OSA 1777 | VitroGel Hydrogel Matrix, VitroGel RGD | spheroid and cluster formation | |
| Glioma RuGli | VitroGel Hydrogel Matrix, VitroGel COL | Integrin dependent cell adhesion | |
| Breast Cancer MCF-7 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, intercellular connections | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation, morphological changes, MMP expression, and angiogenesis | ||
| Liver carcinoma HepG2 | VitroGel Hydrogel Matrix, VitroGel COL | Cell viability, growth, and drug resistance | |
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | ||
| Human pancreatic cancer PANC-1 | VitroGel Hydrogel Matrix, VitroGel RGD | cell proliferation and cellular interations | |
| Primary breast (human) | VitroGel Hydrogel Matrix, VitroGel COL | Cell invasion, migration, and dissemination | |
| Ovarian carcinoma OVCAR-3 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell matrix interations | |
| Ovarian OVCA429 | VitroGel Hydrogel Matrix, VitroGel COL | MMP dependent cell invasion | |
| Human osteosarcoma KHOS | VitroGel Hydrogel Matrix, VitroGel 3D | cell proliferation and spheroids formation | |
| Human osteosarcoma U2OS | VitroGel Hydrogel Matrix, VitroGel 3D | cell proliferation and spheroids formation | |
| Priess human lymphoblastoid cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| Cartilage | Chondrocytes (bovine) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability and proliferation |
| Chondrocytes (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability and proliferation | |
| Connective tissue | Dermal fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability and spreading |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell viability | ||
| Fibroblasts NIH3T3 | VitroGel Hydrogel Matrix, VitroGel RGD | Directional cell migration toward gradient | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading dependent on substrata rigidity | ||
| Foreskin fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell spreading | |
| VitroGel Hydrogel Matrix, VitroGel YIGSR | Cell spreading | ||
| VitroGel Hydrogel Matrix, VitroGel MMP | Substrata degradation and cell invasion | ||
| Skin fibroblasts (skin) | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell adhesion | |
| Epidermal keratinocytes | VitroGel Hydrogel Matrix, VitroGel COL | Cell viability | |
| Epithelial Cells | Mouse ovarian follicle cells | VitroGel Hydrogel Matrix, VitroGel RGD | 3D cell culture using ES-hydrogel can enhance vitro follicle culture by considering the permeability and stiffness of the gel. |
| Human Nthy-ori 3-1 cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| A549 cells | VitroGel Hydrogel Matrix, VitroGel RGD | Enhance cell proliferation and cell matrix interactions. | |
| MCF-12A | VitroGel Hydrogel Matrix, VitroGel RGD | Enhance cell proliferation and cell matrix interactions. | |
| Immortalized bronchial epithelial cells HBEC-KRAS | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Eye | Corneal endothelial B4G12 | VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment and spreading |
| Retinal ganglion cells (xenopus) | VitroGel Hydrogel Matrix, VitroGel COL | Neurite outgrowth | |
| Immune Cells | CD8 + T cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Kidney | Human embryonic kidney HEK293 | VitroGel Hydrogel Matrix, VitroGel RGD | 3D spheroids formation |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and cluster growth | ||
| Madin-Darby Canine Kidney | VitroGel Hydrogel Matrix, VitroGel RGD | Epithelial cysts formation | |
| podocytes (human) | VitroGel Hydrogel Matrix, VitroGel COL | Glomerular capillary formation | |
| glomerular endothelial cells (human) | VitroGel Hydrogel Matrix, VitroGel COL | Glomerular capillary formation | |
| Liver | Hepatocytes (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Filopodia formation and synthesis of albumin |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment | ||
| Hepatocytes (mouse) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability | |
| Hepatocytes (rat) | VitroGel Hydrogel Matrix, VitroGel COL | Albumin secretion | |
| Hepatocytes (swine) | VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading and albumin section | |
| Lung | Alveolar basal epithelial A549 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment |
| Alveolar epithelial RLE-6TN | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment and mesenchymal differentiation | |
| Pulmonary fibroblasts LL2 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell adhesion | |
| HFL1 lung fibroblasts CCL153 | VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and spindle morphology | |
| Lung cancer associated fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel COL | Substrata contractility | |
| Lung fibroblasts MCR-5 | VitroGel Hydrogel Matrix, VitroGel COL | NGF-mediated substrata contraction | |
| Muscle | Myoblasts C2C12 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell Proliferation and differentiation |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment, proliferation, and myofibril formation | ||
| VitroGel Hydrogel Matrix | Myotube formation | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Integrin dependent cell adhesion | ||
| Skeletal myoblasts (mouse) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment, proliferation, and myofibril formation | |
| Myoblasts (human) | VitroGel Hydrogel Matrix, VitroGel COL | Cell adhesion, alignment along fiber, and myotube formation | |
| Myoblasts C25Cl48 | VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation, differentiation and myotube formation | |
| Neural | Dorsal root ganglion (chick) | VitroGel RGD | Neurite formation and outgrowth |
| VitroGel COL | Force dependent neurite outgrowth | ||
| Neural PC12 | VitroGel COL | Neurite outgrowth | |
| VitroGel IKVAV | Neurite outgrowth | ||
| Neural stem cell/progenitor cell (rat) | VitroGel YIGSR | Cell viability | |
| VitroGel IKVAV | Cell attachment and differentiation | ||
| Neural stem cell/progenitor cell (human) | VitroGel IKVAV | Cell viability and differentiation | |
| VitroGel LDP1 | Cell viability and differentiation | ||
| VitroGel LDP1 | Cell viability | ||
| VitroGel COL | Cell attachment | ||
| Schwann cells (rat) | VitroGel YIGSR | Cell attachment and migration | |
| Neural stem cell/progenitor cell (mouse) | VitroGel IKVAV | Cell adhesion and differentiation | |
| Cortical astrocytes (rat) | VitroGel IKVAV | Cell adhesion | |
| Spiral ganglion neurons (mouse) | VitroGel IKVAV | Neurite outgrowth | |
| Motor neurons (human) | VitroGel COL | Force dependent neurite outgrowth | |
| Forebrain neurons (human) | VitroGel COL | Force dependent neurite outgrowth | |
| Cortical neurons (rat) | VitroGel COL | Neuronal viability and neurite outgrowth | |
| Dorsal root ganglion (rat) | VitroGel COL | Neurite outgrowth | |
| Red Blood Cells | Red Blood cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Pancreas | B-cells MIN6 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Reduced apoptosis and increased insulin release |
| Stem cells | Mesenchymal stem cells (human) | VitroGel RGD | Cell viability |
| VitroGel RGD | Cell Proliferation and differentiation | ||
| VitroGel COL | Cell proliferation | ||
| VitroGel IKVAV | Neuronal differentiation | ||
| VitroGel MMP | Neuronal differentiation and neurite outgrowth | ||
| VitroGel COL | Cell attachment, spreading, viability, and osteoblast differentiation | ||
| Mesenchymal stem cells (mouse) | VitroGel RGD | Cell spreading and migration | |
| VitroGel MMP | Cell spreading and migration | ||
| Mesenchymal stem cells (rat) | VitroGel RGD | Cell adhesion and spreading | |
| Embryonic stem cells (mouse) | VitroGel RGD | Endothelial cell differentiation | |
| VitroGel COL | Neuronal differentiation and neurite outgrowth | ||
| VitroGel YIGSR | Neuronal differentiation | ||
| Induced pluripotent stem cells (human) | VitroGel YIGSR | Cell viability | |
| VitroGel IKVAV | Cell viability | ||
| VitroGel LDP1 | Cell viability | ||
| Human Ipsc | VitroGel RGD | Cell proliferation, and cell matrix interactions | |
| Human stem cells from apical papilla SCAP | VitroGel 3D | Cell viability | |
| Adipose derived stem cells (human) | VitroGel IKVAV | Cell attachment | |
| Vascular/cardiac | Umbilical vein endothelial cells (human) | VitroGel RGD | Cell attachment, proliferation, migration, and angiogenesis |
| VitroGel YIGSR | Upregulation in gene expression | ||
| VitroGel IKVAV | Migratory cell infiltration | ||
| VitroGel MMP | Cell attachment, migration, and survival | ||
| VitroGel COL | Cell attachment, spreading, and VEGF dependent migration | ||
| Neonatal cardiac (rat) | VitroGel RGD | Cell attachment and tissue regeneration | |
| VitroGel YIGSR | Cell attachment similar to laminin | ||
| Aortic smooth muscle cells (human) | VitroGel RGD | Cell attachment | |
| Endothelial (human) | VitroGel YIGSR | Cell differentiation | |
| Endotheliocytes | VitroGel YIGSR | Cell migration | |
| Microvascular endothelial cells (human) | VitroGel YIGSR | Cell mobility | |
| Aortic endothelial cells (bovine) | VitroGel COL | Force dependent cell spreading | |
| Capillary endothelial cells (bovine) | VitroGel COL | Capillary like network formation |
Data
Figure 1. Rheological properties of VitroGel COL with DMEM medium.
A) The gel formation curve after mixing with DMEM medium. VitroGel COL was diluted at 1:0,1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with DMEM at 4:1 (v/v) ratio. B) The gel strength after 24 hrs incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test.
Figure 2. 3D culture of mouse bone marrow stromal cells (OP9 mesenchyme) in the mixture of VitroGel COL.
Cells were cultured with 1:5 diluted VitroGel COL The single cells were suspended in hydrogel matrix (Day 1) and extended to form a fibroblast-like cell-matrix structure (Day 7). “
Figure 3. 3D culture of glioblastoma cells (SNB-75) in VitroGel COL.
Cells were cultured with 1:5 diluted VitroGel COL follow the user handbook (50% FBS was used to prepare the cell suspension to get hydrogel with final 10% FBS concentration).
References/Publications
- Powell K. Adding depth to cell culture. Science, 356(6333), 96–98. https://doi.org/10.1126/science.356.6333.96
- References for all VitroGel hydrogels >
| Dilution Solution TYPE | TYPE 1, TYPE 2 |
|---|
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