VitroGel® COL High Concentration
collagen-mimetic functional hydrogel – tunable, xeno-free (3 mL)
VitroGel COL High Concentration
VitroGel COL High Concentration is a tunable, xeno-free hydrogel system that mimics the functions of native collagen. The hydrogel can promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo, which shows great potential for tissue engineering and regeneration medicine application. VitroGel COL High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.
Mix & Match – 3D Cell Culture Your WAY!
Unique to VitroGel High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel® COL can be “mix & matched” with other versions of VitroGel such as VitroGel® RGD, VitroGel® IKVAV, VitroGel® YIGSR, and VitroGel® MMP to create a customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists can customize their 3D culture micro-environment for different applications.
Specifications
| Contents | VitroGel® COL High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
| Hydrogel Formulation | Xeno-free tunable collagen-mimetic functional hydrogel. |
| Use | Support integrin binding to promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo |
| Mix & Match | Can be blended with other versions of VitroGel concentrated hydrogels to create a custom multi-functional matrix. |
| Operation | Room temperature |
| Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
| pH | Neutral |
| Color | Transparent |
| Cell Harvesting | 20 min cell recovery using VitroGel Cell Recovery Solution |
| Injectable | Injectable hydrogel |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Number of Uses | Dilution ratio: 1:2 = 225 uses at 50 µL per well 1:3 = 300 uses at 50 µL per well 1:5 = 450 uses at 50 µL per well |
3D Cell Culture Process in 20 Minutes
VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.
Tunable Hydrogel Strength
Simply diluting the hydrogel controls the gel strength.
Handbooks and Resources
Video Protocols & Demonstrations
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
VIDEO PROTOCOL TIP
Data and References
Cell Type Behavior Reference Table for VitroGel COL
Bone
| Cell Type | Behavior |
|---|---|
| Bovine bone marrow stromal cells | Increased cell spreading and osteocalcin expression |
| Human bone | Increased cell spreading, proliferation, and collagen II production |
| marrow mesenchymal stem cells | |
| Rat bone marrow stromal cells | Increased cell adhesion and osteoblast differentiation |
| Human bone marrow-derived mesenchymal stem cells | Promoted calcium deposition and chondrogenic/ |
| osteogenic differentiation | |
| Mouse bone marrow stromal cells | Supports osteogenesis |
Breast
| Cell Type | Behavior |
|---|---|
| Mouse mammary epithelium | Promoted transient cell invasion and dissemination |
Cancer/Tumor
| Cell Type | Behavior |
|---|---|
| Breast MDA-MB-231 | Increased cell cluster size and spreading |
| Breast T47D | Increased cell cluster size |
| Breast T47D | Promoted force dependent tubule formation |
| Breast MCF-7 | Increased cell proliferation, morphological changes, MMP expression, and angiogenesis |
| Co-culture of liver carcinoma HepG2 and stromal fibroblasts 3T3-J2 | Increased cell viability, growth, and drug resistance |
| Fibrosarcoma HT1080 | Support cell infiltration and growth |
| Fibrosarcoma HT1080 | Promoted integrin dependent cell adhesion |
| Fibrosarcoma HT1080 | Promoted cell adhesion |
| Glioma RuGli | Promoted integrin dependent cell adhesion |
| Glioma U87-MG | Cell migration dependent on mechanical force |
| Prostate PC3 | Increased cell invasion, migration, and spheroid metabolic activity |
| Human primary breast | Promoted cell invasion, migration, and dissemination |
| Melanoma B16F10 | Increased cell migration, invasion, and MMP release |
| Ovarian OVCA429 | MMP dependent cell invasion |
| Prostate LNCaP | Supported cell proliferation and increased prostate-specific antigen release |
| Prostate PC3 | Supported cell proliferation and reduced MMP release |
Connect Tissues
| Cell Type | Behavior |
|---|---|
| Co-culture human dermal fibroblasts and epidermal keratinocytes | Promoted cell viability |
| Fibroblast NIH3T3 | Increased cell spreading on rigid |
Eye
| Cell Type | Behavior |
|---|---|
| Corneal endothelial B4G12 | Increased cell attachment and spreading |
| Xenopus retinal ganglion cells | Promoted neurite outgrowth |
Liver
| Cell Type | Behavior |
|---|---|
| Human Hep3B | Promoted cell attachment |
| Rat hepatocytes | Promote albumin secretion |
| Swine hepatocytes | Promoted cell spreading and albumin section |
Lung
| Cell Type | Behavior |
|---|---|
| Lung fibroblasts HFL1 (CCL153) | Promoted cell proliferation and spindle morphology |
| Human lung cancer associated fibroblasts | Increased smooth muscle actin and substrata contractility |
| Lung fibroblasts MCR-5 | Promoted NGF-mediated substrata contraction |
Muscle
| Cell Type | Behavior |
|---|---|
| Human myoblasts | Promoted cell adhesion, alignment along fiber, and myotube formation |
| Mouse myoblast C2C12 | Promote integrin dependent cell adhesion |
| Myoblasts C2C12 | Promoted formation of myotubes and myotendinous |
| Myoblasts C2C12 | Promote cell proliferation, differentiation, and myotube formation |
| Myoblasts C25Cl48 | Promote cell proliferation, differentiation, and myotube formation |
Neural
| Cell Type | Behavior |
|---|---|
| Human neural stem/progenitor cells | Promoted cell attachment |
| Chick dorsal root ganglion | *Increased neurite length on soft |
| Chick dorsal root ganglion | *Increased neurite outgrowth toward soft |
| Human motor neurons | *Increased neurite length on rigid |
| Human forebrain neurons | *Increased neurite length on soft |
| Neural PC12 | Increased neurite length |
| Rat cortical neurons | Increased neuronal viability and neurite length |
| Rat dorsal root ganglion | Promoted axon outgrowth |
| Rat dorsal root ganglion | Promoted neurite outgrowth |
| Rat spinal cord section | Promoted neurite outgrowth |
Stem Cells
| Cell Type | Behavior |
|---|---|
| Human mesenchymal stem cells | Promoted cell adhesion, spreading, viability, and osteoblast differentiation |
| Human mesenchymal stem cells | Promoted chondrogenic differentiation |
| Human mesenchymal stem cells | Increased cell migration, proliferation, and osteogenic differentiation |
| Human mesenchymal stem cells | Promoted cell attachment and tenogenic differentiation |
| Human mesenchymal stem cells | Promoted cell proliferation |
| Mouse embryonic stem cells | Supported neuronal differentiation and neurite outgrowth |
Vascular
| Cell Type | Behavior |
|---|---|
| Bovine aortic endothelial cells | Promoted cell spreading along fiber |
| Bovine aortic endothelial cells | Increased cell spreading on rigid |
| Bovine capillary endothelial cells | Formation of capillary like networks |
| Human umbilical vein endothelial cells | Increased VEGF dependent vascularization |
| Human umbilical vein endothelial cells | Promoted cell adhesion, spreading and supported increased VEGF dependent migration |
View All Cell Type Behaviors for All VitroGel Products
| TISSUE/ORGAN TYPE | CELL TYPE | READY TO USE | HIGH CONCENTRATION | BEHAVIOR |
|---|---|---|---|---|
| Beta Cell | BL5 human beta cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel 3D, VitroGel MMP | Enhance spheroids formation |
| Beta TC3 cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and cellular interactions | |
| Bone | Bone marrow stromal cells (rat) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel MMP | Osteogensic differentiation , Cell attachment and osteoblast differentiation,Cell proliferation, cell viability, and cellular networking. |
| Osteoblasts (rat) | VitroGel MSC, VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell attachment and spreading | |
| Bone marrow stromal cells (bovine) | VitroGel MSC, VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell spreading and osteocalcin expression | |
| Breast | Mammary gland MCF10A | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitrolGel RGD, VitroGel COL, VitroGel MMP | Spheroid formation, MMP activity in response to TGF-B1 |
| Mammary epithelium (mouse) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell invasion and dissemination | |
| Cancer/Tumor | Human colorectal carcinoma HCT 116 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation, cell survival, and intercellular networking |
| Huaman colon carcinoma HCT-8 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and cell matrix interaction | |
| Glioma U87-MG | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel MMP, VitroGel COL | Cell spreading and acting stress fiber assembly, cell proliferation, spreading, and migration, Cell migration dependent on mechancial force, Cell proliferation and cell matrix interaction | |
| Gliobastoma SF 268 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitrolGel RGD | Cell proliferation and cell matrix interaction | |
| Gliobastoma SF 295 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and cell matrix interaction | |
| Glioblastoma SNB75 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and cell matrix interaction | |
| Glioblastoma U-251 MG | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and cell matrix interaction | |
| Prostate PC3 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel COL, VitroGel IKVAV, VitroGel RGD, VitroGel MMP | Cell proliferation, reduced MMP release, invasion, migration, and spheroid metabolic activity. | |
| Prostate LNCaP | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell attachment, proliferation, and prostate specific antigen release | |
| Prostate CRPC | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and invasion | |
| Prostate DU145 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and invasion | |
| Melanoma B16F10 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel COL, VitroGel YIGSR | Cell migration, invasion, MMP release, cell attachment and spreading | |
| Breast MDA-MB-231 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel MMP, VitroGel 3D | Cell invasion, spreading, proliferation, division, migration, and cluster growth | |
| Fibrosarcoma HT1080 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell infiltration, attachment | |
| Breast T47D | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel COL, VitroGel 3D, VitroGel RGD, VitroGel MMP | Force dependent tubule formation, cell cluster growth, spheroid formation and proliferation | |
| Breast 4T1 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation | |
| Breast CTC | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel 3D, VitroGel RGD | Cell proliferation | |
| Breast E0771 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation spheroid formation | |
| Brest AU-565 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation cell matrix interactions | |
| Epithelial ovarian OV-MZ-6 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Spheroid formation and proliferation | |
| Epithelial ovarian SKOV-3 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Spheroid formation and proliferation | |
| Glioma U373-MG | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel MMP | Cell adhesion, invasion and migration | |
| Rhabdomyosarcoma (human) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel YIGSR | Cell attachment and spreading | |
| Melanoma SK-MEL-28 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel IKVAV | Cell adhesion and proliferation | |
| Melanoma K-1735 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel IKVAV | Cell invasion | |
| Melanoma A2058 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel IKVAV | Collagenolytic activity | |
| Brainstem glioma DIPG | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel MMP | Cell proliferation and survival | |
| Hela Cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel 3D, VitroGel RGD, VitroGel MMP | Cell proliferation | |
| Colorectal adenocarcinoma DLD-1 cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and cell matrix interaction | |
| Giloma LRM55 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel IKVAV, VitroGel MMP | Cell attachment | |
| Melanoma WM 239A | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel MMP | Cell invasion | |
| Melanoma Cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and cell matrix interaction | |
| Insulinoma ins-1 (Rat) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and cell matrix interaction | |
| Biphasic synovial sarcoma SYO-1 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation, cell matrix interation, and cell survival | |
| Fuji Cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and cell matrix interaction | |
| Chordoma Cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel 3D | Cell proliferation | |
| Bone OSA 1777 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Spheroid and cluster formation | |
| Glioma RuGli | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel COL | Integrin dependent cell adhesion | |
| Breast Cancer MCF-7 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel MMP, VitroGel 3D | Cell proliferation, intercellular connections, morphological changes, MMP expression, and angiogenesis | |
| Liver carcinoma HepG2 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell viability, growth, drug resistance, proliferation, and cellular matrix interaction | |
| Human pancreatic cancer PANC-1 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel MMP | Cell proliferation and cellular interactions | |
| Primary breast (human) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel MMP | Cell invasion, migration, and dissemination | |
| Ovarian carcinoma OVCAR-3 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel MMP | Cell proliferation, cell matrix interactions | |
| Ovarian OVCA429 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel MMP, VitroGel COL | MMP dependent cell invasion | |
| Human Osteosarcoma KHOS | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel 3D | Cell proliferation and spheroids formation | |
| Human Osteosarcoma U2OS | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel 3D | Cell proliferation and spheroids formation | |
| Priess human lymphoblastoid cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| Cartilage | Chondrocytes (bovine) | VitroGel MSC, VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell viability and proliferation |
| Chondrocytes (human) | VitroGel MSC, VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell viability and proliferation | |
| Connective Tissue | Dermal Fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell viability and spreading |
| Fibroblasts NIH3T3 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Directional cell migration toward gradient and cell spreading dependent on substrata rigidity | |
| Foreskin fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel YIGSR, VitroGel MMP | Cell spreading, substrata degradation, and cell invasion | |
| Skin fibroblasts (skin) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel IKVAV | Cell adhesion | |
| Epidermal keratinocytes | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell viability | |
| Epithelial Cells | Mouse ovarian follicle cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | 3D cell culture using ES-hydrogel can enhance vitro follicle culture by considering the permeability and stiffness of the gel. |
| Human Nthy-ori 3-1 cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| A549 cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Enhance cell proliferation and cell matrix interactions. | |
| MCF-12A | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Enhance cell proliferation and cell matrix interactions. | |
| Immortalized bronchial epithelial cells HBEC-KRAS | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel 3D | Cell proliferation | |
| Eye | Corneal endothelial B4G12 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit, VitroGel Angiogenesis Assay | VitroGel RGD, Vitrogel Angiogenesis Assay HC kit | Cell attachment and spreading |
| Retinal ganglion cells (xenopus) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Neurite outgrowth | |
| Immune Cells | CD8 + T cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Kidney | Human embryonic kidney HEK293 | VitroGel Hydrogel Matrix, VitroGel HEK293, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | 3D spheroids formation |
| Madin-Darby Canine Kidney | VitroGel Hydrogel Matrix, VitroGel HEK293, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel MMP | Epithelial cysts formation | |
| Podocytes (human) | VitroGel Hydrogel Matrix, VitroGel HEK293, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Glomerular capillary formation |
|
| glomerular endothelial cells (human) | VitroGel Hydrogel Matrix, VitroGel HEK293, VitroGel ORGANOID Kit, VitroGel Angiogenesis Assay | VitroGel RGD, Vitrogel Angiogenesis Assay HC kit | Glomerular capillary formation |
|
| Liver | Hepatocytes (human) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Filopodia formation and synthesis of albumin and cell attachment |
| Hepatocytes (mouse, rat, swine) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel MMP | Cell viability, spearding, Albumin secretion | |
| Lung | Alveolar basal epithelial A549 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell attachment |
| Alveolar epithelial RLE-6TN | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell attachment and mesenchymal differentiation | |
| Pulmonary fibroblasts LL2 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel IKVAV | Cell adhesion | |
| HFL1 lung fibroblasts CCL153 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell proliferation and spindle morphology | |
| Lung cancer associated fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Substrata contractility | |
| Lung fibroblasts MCR-5 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | NGF-mediated substrata contraction | |
| Muscle | Myoblasts C2C12 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell proliferation, differentiation, attachment, myofibril formation, myotube formation, and integrin dependent cell adhesion |
| Skeletal myoblasts (mouse) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD | Cell attachment, proliferation, and myofibril formation | |
| Myoblasts (human) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell adhesion, alignment along fiber, and myotube formation | |
| Myoblasts C25Cl48 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Cell proliferation, differentiation and myotube formation | |
| Neural | Dorsal root ganglion (chick) | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL | Neurite formation and force dependent neurite outgrowth |
| Neural PC12 | VitroGel Hydrogel Matrix | VitroGel RGD, VitroGel IKVAV | Neurite outgrowth | |
| Neural stem cell/ progenitor cell (rat) | VitroGel STEM, VitroGel Hydrogel Matrix | VitroGel RGD, VitroGel IKVAV | Cell viability, attachment, and differentiation | |
| Neural stem cell/ progenitor cell (human) | VitroGel STEM, VitroGel Hydrogel Matrix | VitroGel RGD, VitroGel IKVAV, VitroGel COL | Cell viability, attachment, and differentiation | |
| Schwann cells (rat) | VitroGel Hydrogel Matrix | VitroGel RGD | Cell attachment and migration | |
| Neural stem cell/ progenitor cell (mouse) | VitroGel STEM | VitroGel RGD, VitroGel IKVAV | Cell adhesion and differentiation | |
| Cortical astrocytes (rat) | VitroGel Hydrogel Matrix | VitroGel RGD, VitroGel IKVAV | Cell adhesion | |
| Spiral ganglion neurons (mouse) | VitroGel Hydrogel Matrix | VitroGel RGD, VitroGel IKVAV | Neurite outgrowth | |
| Motor neurons (human) | VitroGel Hydrogel Matrix | VitroGel RGD, VitroGel COL, VitroGel IKVAV | Force dependent neurite outgrowth | |
| Forebrain neurons (human) | VitroGel Hydrogel Matrix | VitroGel RGD, VitroGel COL, VitroGel IKVAV | Force dependent neurite outgrowth | |
| Cortical neurons (rat) | VitroGel Hydrogel Matrix | VitroGel RGD, VitroGel COL, VitroGel IKVAV | Neuronal viability and neurite outgrowth | |
| Dorsal root ganglion (rat) | VitroGel Hydrogel Matrix | VitroGel RGD, VitroGel COL, VitroGel IKVAV | Neurite outgrowth | |
| Red Blood Cells | Red Blood Cells | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel 3D | Enhance Spheroids and cluster formation and promote cell viability |
| Pancreas | B-cells MIN6 | VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel IKVAV | Reduced apoptosis and increased insulin release |
| Stem Cells | Mesenchymal stem cells (human) | VitroGel MSC | VitroGel RGD, VitroGel COL, VitroGel IKVAV, VitroGel MMP | Cell viability, proliferation, differentiation, neuronal differntiation, neurite outgrowth, attachment, spreading, viability, and osteoblast differentiation |
| Mesenchymal stem cells (mouse) | VitroGel MSC | VitroGel RGD, VitroGel MMP | Cell spreading and migration | |
| Mesenchymal stem cells (rat) | VitroGel MSC | VitroGel RGD | Cell adhesion and spreading | |
| Embryonic stem cells (mouse) | VitroGel STEM, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel COL, VitroGel YIGSR | Endothelial cell differentiation, neuronal differentiation, and neurite outgrowth | |
| Induced pluripotent stem cells (human) | VitroGel STEM, VitroGel ORGANOID Kit | VitroGel RGD, VitroGel YIGSR, VitroGel IKVAV | Cell viability | |
| Human stem cells from apical papilla SCAP | VitroGel STEM | VitroGel RGD | Cell viability | |
| Hematopoietic Stem Cells | VitroGel STEM | Cell viability | ||
| Adipose derived stem cells (human) | VitroGel MSC | VitroGel RGD, VitroGel 3D, VitroGel IKVAV | Cell viability, cell attachment | |
| Vascular/cardiac | Umbilical vein endothelial cells | Vitrogel Angiogenesis Assay , VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | Vitrogel Angiogenesis Assay HC kit | Cell attachment, proliferation, migration, angiogenesis, gene expression changes, migratory cell infiltration, cell survival, and VEGF dependent migration |
| Neonatal cardiac (rat) | Vitrogel Angiogenesis Assay, VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit | Vitrogel Angiogenesis Assay HC kit | Cell attachment, tissue regeneration, and attachment similar to laminin | |
| Aortic smooth muscle cells | VitroGel Hydrogel Matrix | Vitrogel Angiogenesis Assay HC kit | Cell attachment | |
| Endothelial (human) | VitroGel Angiogenesis Assay | Vitrogel Angiogenesis Assay HC kit | Cell differentiation | |
| Endotheliocytes | VitroGel Angiogenesis Assay | Vitrogel Angiogenesis Assay HC kit | Cell migration | |
| Microvascular endothelial cells (human) | VitroGel Angiogenesis Assay | Vitrogel Angiogenesis Assay HC kit | Cell mobility | |
| Aortic endothelial cells (bovine) | VitroGel Angiogenesis Assay | Vitrogel Angiogenesis Assay HC kit | Force dependent cell spreading | |
| Capillary endothelial cells (bovine) | VitroGel Angiogenesis Assay | Vitrogel Angiogenesis Assay HC kit | Capillary like network formation |
Data
Figure 1. Rheological properties of VitroGel COL with DMEM medium.
A) The gel formation curve after mixing with DMEM medium. VitroGel COL was diluted at 1:0,1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with DMEM at 4:1 (v/v) ratio. B) The gel strength after 24 hrs incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test.
Figure 2. 3D culture of mouse bone marrow stromal cells (OP9 mesenchyme) in the mixture of VitroGel COL.
Cells were cultured with 1:5 diluted VitroGel COL The single cells were suspended in hydrogel matrix (Day 1) and extended to form a fibroblast-like cell-matrix structure (Day 7). “
Figure 3. 3D culture of glioblastoma cells (SNB-75) in VitroGel COL.
Cells were cultured with 1:5 diluted VitroGel COL follow the user handbook (50% FBS was used to prepare the cell suspension to get hydrogel with final 10% FBS concentration).
References/Publications
- Powell K. Adding depth to cell culture. Science, 356(6333), 96–98. https://doi.org/10.1126/science.356.6333.96
- References for all VitroGel hydrogels >
| High Concentration Kit Type | VitroGel COL + Dilution Solution TYPE 1, VitroGel COL + Dilution Solution TYPE 2 |
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