3D Cell Culture


Closely mimicking the natural extracellular matrix (ECM) environment, our state-of-the-art hydrogel system gives an outstanding balance of biological functions and operating ease to establish a robust 3D cell culture platform and injectable delivery system to advance drug discovery, tissue engineering, cell therapy, and personalized medicine.

Animal-based basement membrane has known batch-to-batch inconsistency and with over 2,000 undefined components. In contrast, the VitroGel system is a defined synthetic hydrogel with functional ligand modifications with no animal or human compounds. The VitroGel system provides robust hydrogel properties with controllable mechanical strength, functional ligands, & degradability. Researchers have control of the whole hydrogel system to create a biomimicking environment (ECM) for many different applications.

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A xeno-free system is key for 3D cell culture in clinical applications. Our cutting-edge hydrogel enables high-throughput capable processing to establish 3D organotypic models from both cell lines and patient-derived cells. Using our hydrogel system as an injectable delivery system, scientists can achieve better cell retention and higher cell viability for cell therapy.  This groundbreaking technology allows for cell recovery from the 3D hydrogel matrix easier than ever before. Our products can be manufactured under cGMP and open the door for clinical applications.

VitroGel Hydrogel Features
Closely mimicking the natural extracellular matrix (ECM) environment, this distinct system brings many advantages to bridge in vitro and in vivo studies through 3D cell culture and beyond.

VitroGel® Ready-To-Use Hydrogels
A series of user-friendly functional hydrogels offering an excellent balance of simplicity and versatility.

The ready-to-use VitroGel hydrogels have optimized multi-functional ligands and concentrations formulations to support a wide range of cell types for different applications, including various 3D cell models, stem cell spheroid, and organoids. Each hydrogel is ready to mix with cell suspension directly. There is no additional adjustment required. For many users, this is the preferred hydrogel for 3D cell culture and 2D coating.

VIew Ready-To-Use Hydrogels

VitroGel® High Concentration Hydrogels
Tunable Hydrogels – Building blocks for unlimited creativity and maximum flexibility

VitroGel High Concentration Hydrogels offer a set of building blocks to create a functional micro-environment that closely mimics the natural extracellular matrix (ECM) for cells to feel like at home. The high concentration formulation allows the maximum flexibility to manipulate the mechanical strength of hydrogel from 10 to 4000 Pa. The hydrogels come with several types of functional ligand modifications such as RGD (VitroGel RGD), Collagen (VitroGel COL), Laminin (VitroGel IKVAV & VitroGel YIGSR), and biodegradable Matrix Metalloproteinase (VitroGel MMP). The system allows scientists to investigate the cell behaviors in response to an individual functional ligand or create multi-functional hydrogel combinations by blending different versions of VitroGel.

Create your own ECM.  Create over 100 possible combinations using the high concentration hydrogels.

Learn How >

View tunable high concentration hydrogels

multiple cell culture methods

The XENO-FREE bio-functional VitroGel hydrogel system is versatile for many applications. There are multiple ways to use our hydrogel system to fulfill many research needs. Here are five of our most popular cell culture methods: 3D cell culture, 2D hydrogel coating, static suspension culture, hydrogel-cell bead, and as an injectable carrier. These five culture methods apply to all our ready-to-use VitroGel and high concentration VitroGel systems.

Learn more about multiple cell culture methods >

Easy Cell Harvesting from Hydrogel system

As easy as it is to perform 3D or 2D cell culture with VitroGel, it is even easier to harvest cells out from the hydrogel system with the VitroGel® Organoid Recovery Solution. This enzyme-free solution has a neutral pH and works at 37°C operating temperature. With a simple rocking and incubation process, cells cultured in 3D or 2D coating VitroGel can be harvested within 15 minutes while maintaining high cell viability.

Learn more on how cell recovery works >

15 min protocol

An Injectable hydrogel for multiple applications

VitroGel hydrogel system has a unique shear-thinning and rapid recovery rheological property. Under the mechanical shearing force, such as injection through a syringe, the hydrogel performs a gel-sol transition and becomes free-flowing status. However, once the shearing force ceased, the mechanical strength of the hydrogel can rapidly recover with a sol-gel transition and become a hydrogel status again.

With this injectable property, VitroGel can be used for in vivo cells/drug delivery with great retention, bioprinting, droplet formation, and laboratory automation.

Performance Comparison to other 3d cell culture methods

See how VitroGel features compare to other 3D cell culture methods above.

VitroGel gives an outstanding balance of biological functions and operating feasibility. The system can closely mimic the natural extracellular matrix and allow the high-throughput performance to build robust 3D cell models.

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