Applicable To The Following Products:
VHM01 – VitroGel Hydrogel Matrix
VHM02 – VitroGel STEM
VHM03 – VitroGel MSC
VHM04 – VitroGel ORGANOID
VHM05 – VitroGel HEK293
Protocols and Guidelines
View handbook and guidelines for our products. If you have any questions, email support@thewellbio.com
UNIVERSAL PROTOCOLS
Xenograft – Injection Protocol Five Cell Culture Methods – Ready to Use VitroGel Five Cell Culture Methods – High Concentration VitroGel
DOWNSTREAM PROTOCOLS
Cell Recovery from Gel Immunofluorescence Staining Histological Analysis/Sample Preparation Protocol Cell Fluorescent Staining in Gel for Nuclei and Actin Filament Cell Proliferation Assay RNA/DNA Extraction, Cell Lysis Protocol Cell Viability Live-Dead Assay Protocol
INDIVIDUAL PRODUCT PROTOCOLS
READY-TO-USE HYDROGELS
BIOINKS
Video Protocols
VIDEO PROTOCOL TIP
3D Cell Culture
“Ready-To-Use” Hydrogels
VIDEO PROTOCOL TIP
2D Coating Culture
“Ready-To-Use” Hydrogels
VIDEO PROTOCOL TIP
Cell Recovery Method 1
3D & 2D
VIDEO PROTOCOL TIP
Cell Recovery Method 2
3D & 2D
VIDEO PROTOCOL TIP
Injectable Hydrogel
VitroGel System
Additional To VitroGel STEM
VIDEO PROTOCOL TIP
hPSC Suspension Culture with
VitroGel STEM
VIDEO PROTOCOL TIP
hPSC Spheroids
Cell Harvesting
Cultured in VitroGel STEM
Additional To VitroGel ORGANOID
VIDEO PROTOCOL TIP
3D Organoid Culture
VIDEO PROTOCOL TIP
Harvesting
3D Organoid
Additional To VitroGel MSC
VIDEO PROTOCOL TIP
MSC 3D Culture
Scale-up
Bead Droplet Method
VIDEO PROTOCOL TIP
Harvesting MSC
from 3D Culture Scale-up
Additional To VitroGel HEK293
VIDEO PROTOCOL TIP
HEK293 3D Suspension Culture
VIDEO PROTOCOL TIP
HEK293 Suspension Cell Harvesting
Applicable To The Following Products:
TWG001 – VitroGel 3D High Concentration
TWG003 – VitroGel RGD High Concentration
TWG007 – VitroGel IKVAV High Concentration
TWG008 – VitroGel YIGSR High Concentration
TWG009 – VitroGel COL High Concentration
TWG010 – VitroGel MMP High Concentration
VIDEO PROTOCOL TIP
3D Cell Culture
VitroGel High Concentration Hydrogels
VIDEO PROTOCOL TIP
2D Cell Coating
VitroGel High Concentration Hydrogels
VIDEO PROTOCOL TIP
Cell Recovery
Method 1
from 3D & 2D cultures
VIDEO PROTOCOL TIP
Cell Recovery
Method 2
from 3D & 2D cultures
VIDEO PROTOCOL TIP
Injectable Hydrogel
VitroGel System
